福建省2009年甲型H1N1流感病毒NA基因特征及对达菲的耐药性

目的监测福建省甲型H1N1流感病毒NA基因的变化以及对达菲的耐药情况,为临床诊疗和疾病控制提供参考依据。方法从福建省流感监测网络中随机选取23株甲型H1N1流感病毒,经病毒核酸提取和一步法RT-PCR扩增,获得NA基因片段,双向测定核苷酸序列,分析NA基因序列和重要氨基酸位点特征。结果 23株甲型H1N1流感病毒NA片段基因与A/California/07/2009(H1N1)代表株的核苷酸序列进行比较,同源性高达98.1%以上;23株毒株NA蛋白第275位氨基酸均为组氨酸。结论随机选取的23株甲型H1N1流感病毒NA基因片段保持高度同源并且对达菲仍然敏感。随着国内外达菲耐药株的不断出现,应加强耐药性监测,为制定应对甲型H1N1流感流行措施提供参考。

Preliminary study on characteristics of NA genes and oseltamivir resistance in 2009 pandemic H1N1 influenza viruses in Fujian, China

To investigate characteristics of NA genes and oseltamivir susceptibility of 2009 pandemic H1N1 influenza viruses in Fujian Province, twenty-three strains were randomly selected for detection. Fragments of NA genes were amplified by one-step RT-PCR and then were sequenced. Comparing with the A/California/OT/2OO9（H1N1）, alignment of nucleic acid sequences revealed a high homology of 98.1%. While the amino acid residuals at 275 remained histidine, indicating all of the 23 isolates were oseltamivir-sensitive. This observation suggests no oseltamivir-resistant strains has emerged in Fujian. However, continuous surveillance is necessary for control and prevention strategies of the ongoing pandemic.