| 应用量子点及磁微粒技术快速检测阴道毛滴虫抗原 |
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| 引用本文： | 李蒙,蒲晓允,李玲,余娟春. 应用量子点及磁微粒技术快速检测阴道毛滴虫抗原[J]. 第三军医大学学报, 2009, 31(8): 740-743 |
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| 作者姓名： | 李蒙 蒲晓允 李玲 余娟春 |
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| 作者单位： | 第三军医大学新桥医院检验科,重庆,400037;第三军医大学新桥医院检验科,重庆,400037;第三军医大学新桥医院检验科,重庆,400037;第三军医大学新桥医院检验科,重庆,400037 |
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| 基金项目： | 重庆市人口和计划生育委员会资助项目 |
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| 摘 要： | 目的 以阴道毛滴虫(trichomonas vaginalis,Tv)检测为切入点,构建以量子点和磁微粒技术为基础的夹心法快速检测抗原技术,为同步多项抗原检测作准备.方法 针对Tv特异性黏附蛋白AP33制备其抗体,以碳二亚胺交联法,分别连接抗体.量子点及抗体-磁微粒加入待测抗原AP33结合后,在荧光显微镜下观测结果.结果 确立了本检测系统的最优条件,对AP33的检测与儿种阴道常见病原菌无交叉反应,检测限为0.05υg/ml.结论 成功构建了量子点磁微粒检测技术,对Tv抗原检测,特异度为90%,准确率为88%.
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| 关 键 词： | 量子点 磁微粒 阴道毛滴虫 抗原检测 |
Rapid detection of Trichomonas vaginalis antigens by quantum dots and magnetic beads |
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| LI Meng,PU Xiao-yun,LI Ling,YU Juan-chun. Rapid detection of Trichomonas vaginalis antigens by quantum dots and magnetic beads[J]. Acta Academiae Medicinae Militaris Tertiae, 2009, 31(8): 740-743 |
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| Authors: | LI Meng PU Xiao-yun LI Ling YU Juan-chun |
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| Affiliation: | LI Meng,PU Xiao-yun,LI Ling,YU Juan-chun(Department of Laboratory Medicine,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,China) |
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| Abstract: | Objective To establish a new double-antibody sandwich ELISA to detect the antigen AP33(a 33 kDa adhesive protein) of Trichomonas vaginalis based on the quantum dots and magnetic beads.Methods After BALB/c mice were immunized by AP33,the multiclonal antibodies in the antiserum was conjugated with the quantum dots and magnetic beads by carbon diimine crosslinking method respectively.Then the antibodies combined with magnetic beads were coated to microwell of plate as capturing antibody,and the antibodies boun... |
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| Keywords: | quantum dots magnetic beads Trichomonas vaginalis antigen detection |
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