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Antigenic analysis of intraepidemic variants of influenza A (H3N2) viruses by hyperimmune rat antisera |
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| Authors: | R Pyh?l? L Pyh?l? |
| Institution: | 1. Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, Seoul, South Korea;2. KHAV Co., Ltd., 1 Hwayang-dong, Gwangjin-gu, Seoul, Korea;3. Southeast Poultry Research Laboratory, U.S. National Poultry Research Center, U.S. Department of Agriculture-Agricultural Research Service, 934 College Station Road, Athens, GA 30605, USA;4. Department of Microbiology, College of Medicine, Chungbuk National University, Cheongju, South Korea;1. QuadMedicine Inc., Seongnam, Gyeonggi 13209, Republic of Korea;2. Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul 03760, Republic of Korea;1. Faculty of Hepato-Pancreato-Biliary Surgery, Institute of Hepatobiliary Surgery, The First Medical Center, Chinese PLA General Hospital, Beijing, 100853, China;2. The Fifth Medical Center, Chinese PLA General Hospital, Beijing, 100039, China;3. School of Basic Medicine, Inner Mongolia Medical University, Hohhot, China |
| Abstract: | Hyperimmune rat antisera prepared against 5 recent antigenic variants of influenza A (H3N2) viruses were studied for haemagglutination inhibiting (HI) antibodies to the homologous and the heterologous viruses. The ratios of homologous to heterologous reactions varied from one animal to another in immunizations with each of the immunogens. Some antisera exhibited a ratio high enough to allow differentiation of the epidemic variants and demonstration of an intraepidemic heterogeneity of field strains isolated during the outbreak of 1985/86. The variation of cross-reactions of polyclonal antisera may reflect differences in the range of specificities of anti-haemagglutinin antibodies produced by individual animals. The significance of this finding in the classification of influenza A (H3N2) viruses is discussed. Lack of nonspecific inhibitors interfering with the HI test is an additional advantage of hyperimmune rat antisera in typing influenza A and B virus isolates. |
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