2011年龙岩市手足口病例肠道病毒通用引物核酸阳性未分型样本病原分析

目的 了解龙岩市大量判定为其他肠道病毒（EV）感染的手足口病（HFMD）样本的病原组成。方法 对龙岩市2011年经Real-time RT-PCR检测判为未分型EV感染的104份HFMD咽拭子样本经RD细胞分离培养后以RT-PCR法检测EV、EV71、CoxA16,对仍不能分型的样本以187/222引物进行扩增,对扩增产物进行纯化回收、克隆、测序,并用Blast程序在线比对判定型别。同时对培养物进行Real-time RT-PCR检测EV、EV71、CoxA16。任一法检出培养物阳性即判为阳性。结果 104份样本中检出EV阳性88份（84.62%）,包括29份EV71,4份EV71＋CoxA16,3份CoxB5,2份CoxA10,50份仍未能分型。结论 Real-time RT-PCR检测未能分型的HFMD病例中,相当部分（37.18%）还是由EV71所致,临床不应该随意排除该类病例EV71感染的可能,同时还包含CoxB5和CoxA10病毒。

Pathogenic Analysis on HFMD Cases of Enterovirus Universal Primer Nucleic Acid Positive Unclassified Samples,Longyan City, 2011

Objective To understand the pathogens of Hand, Foot and Mouth Disease （HFMD） samples identified as other Enterovirus （EV） infection in Longyan city. Methods Totally 104 HFMD samples identified as other EV infection by Real-time RT-PCR were collected and cultured on RD cells for virus isolation,then use RT-PCR to recheck virus types after isolation. For those samples that still cannot be typed, use primers 187/222 for amplification. PCR products were collected for sequencing and online alignment by Blast. Results Eighty-eight（84.62%） EV nucleic acid positive results were detected in all 104 samples,including EV71（29） ,EV71＋ CoxA16（4） ,CoxBS（3） and CoxA10（2） ,50 samples still cannot be classified. Conclusion A considerable part of HFMD samples which cannot be typed by Real-time RT PCR are caused by EV71 ,so EV71 as well as CoxB5 and CoxA10 infection should not be excluded randomly in clinical patients.