| 前胡丙素对牛主动脉平滑肌细胞增殖的抑制作用 |
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| 引用本文： | Wei EH,Rao MR,Chen XY,Fan LM,Chen Q. 前胡丙素对牛主动脉平滑肌细胞增殖的抑制作用[J]. Acta pharmacologica Sinica, 2002, 23(2): 129-132 |
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| 作者姓名： | Wei EH Rao MR Chen XY Fan LM Chen Q |
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| 作者单位： | 南京医科大学心血管药理研究室,南京医科大学心血管药理研究室,南京医科大学动脉粥样硬化研究中心,南京医科大学动脉粥样硬化研究中心,南京医科大学动脉粥样硬化研究中心 南京,中国 210029,南京,中国 210029,南京,中国 210029,南京,中国 210029,南京,中国 210029 |
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| 基金项目： | Project supported by the National Natural Science Foundation of China, № 39870363. |
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| 摘 要： | 目的:探讨前胡丙素(pra-C)对牛主动脉平滑肌细胞(SMC)增殖的影响 方法:细胞DNA的合成量由~3H-胸腺嘧啶核苷[~3H]TdR)掺入试验测定,应用流式细胞术测定细胞周期,用乳酸脱氢酶活性测定观察药物的毒性.结果:无论有无血管紧张素Ⅱ(AngⅡ),在0.01μmol/L到10μmol/L浓度范围内,pra-C均可呈浓度依赖性地抑制SMC的增殖,抑制作用主要与阻止细胞进入有丝分裂期有关,而与细胞毒性无关.结论:pra-C能够完全阻断Aug Ⅱ诱导SMC的增殖效应,部分阻止小牛血清诱导的细胞分裂,这对于血管增生性疾病的防治有重要意义.
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| 关 键 词： | 前胡丙素 牛 主动脉 平滑肌 细胞分裂 |
Inhibitory effects of praeruptorin C on cattle aortic smooth muscle cell proliferation |
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| Wei En-Hui,Rao Man-Ren,Chen Xiu-Ying,Fan Le-Ming,Chen Qi. Inhibitory effects of praeruptorin C on cattle aortic smooth muscle cell proliferation[J]. Acta pharmacologica Sinica, 2002, 23(2): 129-132 |
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| Authors: | Wei En-Hui Rao Man-Ren Chen Xiu-Ying Fan Le-Ming Chen Qi |
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| Affiliation: | Department of Cardiovascular Pharmacology, Nanjing Medical University, Nanjing 210029, China. |
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| Abstract: | AIM: To study the effects of praeruptorin C (pra-C) on proliferation of cattle aortic smooth muscle cells (SMC). METHODS: The DNA synthesis of SMC was measured using the incorporation of [3H]thymidine([3H]TdR). Cell cycle phase was evaluated by flow cytometry and cytotoxicity was evaluated by measuring lactic dehydrogenase (LDH) activity. RESULTS: Whether or not treated with angiotensin II (Ang-II), SMC proliferation was suppressed by pra-C in a concentration-dependent manner at range from 0.001 micromol/L to 10 micromol/L. The inhibitory effects appeared to be related to G1-S block in cell cycle traverse while the LDH activities did not change dramatically. CONCLUSION: Pra-C can completely inhibit SMC proliferation induced by Ang II and partly inhibit the growth of SMC- induced by bovine serum, which is important in prevention and treatment of vascular hyperplastic disease. |
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| Keywords: | praeruptorin C cattle aorta smooth muscle cell division |
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