冻存增强人外周血单个核细胞产生IL－2的机理初探

作者用ＥＬＩＳＡ法研究了冻存对人外周血革个核细胞（ＰＢＭＣ）产生白介素２（ＩＬ－２）的影响。结果表明，冻存后ＰＢＭＣ产生ＩＬ－２能力明显增强；去除ＣＤ８＋细胞或加入消炎痛后，ＰＢＭＣ产生ＩＬ－２能力增强，且与ＰＢＭＣ冻存后产生ＩＬ－２水平相同。研究提示：冻存过程有可能通过抑制ＰＧＥ２分泌细胞及使ＣＤ８＋细胞失活，使ＰＢＭＣ增加ＩＬ－２的产生。

STUDY ON MECHANISMS OF CRYOPRESERVATION-INDUCED ENHANCEMENT OF INTERLEUKIN-2 PRODUCTION IN MONONUCLEAR CELLS OF HUMAN PERIPHERAL BLOOD

To better understand the effect of cryopreservation on various immunocompetent cell functions,we have examined the interleukin-2 (IL-2) producing activities of frozen mononuclear cells (PBMC) from healthy subjects. The level of IL-2 in culture supernatants of PBMC was assayed by the ELISA method.The results showed that the frozen PBMC produced significantly larger quantities of IL-2than fresh cells. Similar to freezing,indomethacin treatment also increased the IL-2 producing activities of fresh cells. but could no longer enhance the production of IL-2 by frozen cells. Elimination of CD8 T cells from PBMC also resulted in the increase of IL-2 by fresh cells and freezing did not further enhance the IL-2 secreting activities of PBMC, which were devoid of CD8 T cells. These data indicate that inactivation of CD8 T cells and inhibition of PGE2 secreting cells during the freezing procedure are responsible for the increase in IL-2 secretion.