Fas及其配体FasL在自身免疫性甲状腺疾病中的表达

目的研究Graves病(GD)和桥本氏甲状腺炎(HT)组织中细胞凋亡有关的Fas及其配体FasL的表达情况,探讨它们在自身免疫性甲状腺疾病发病机制中的作用。方法采用免疫组化技术,检测Fas蛋白及FasL在65例GD、47例HT和15例正常甲状腺组织中的表达情况。结果 Fas在GD和HT的甲状腺组织中的表达程度均明显高于在正常甲状腺中的表达,且在HT中的表达程度比在GD明显增强。FasL在GD和HT的甲状腺组织中的表达程度均明显高于在正常甲状腺中的表达,且在HT中的表达程度比在GD明显增强。在GD和HT中Fas与FasL的表达程度呈正相关。结论从正常到GD再到HT,Fas/FasL的表达逐渐增高,说明Fas/FasL在自身免疫性甲状腺疾病发展过程中起重要作用。GD和HT的甲状腺滤泡上皮细胞均可表达Fas及其配体FasL,提示甲状腺上皮细胞上Fas和FasL的相互作用可能是甲状腺中自身免疫性排斥的主要机制,也可能是自身免疫性疾病的共同的病理生理基础。

The role of Fas/FasL in the patbogenesis of autoimmune thyroid disease

Objective To investigate the expression of key molecules regulating cell death (Fas, Fas ligand FasL]) in thyrocytes from patients with Graves' Disease (GD) and Hashimoto ' s Thyroiditis (HT) . To explore the role of them in the pathogenesis of autoimmune thyroid disease. Methods The expression of FasL was detected with Power Vision Two - Step immunohistochemical method in thyroid glands from 65 patients with GD, 47 patients with HT and 15 Cases of normal controls, and the express of Fas was detected with S - P immunohistochemical method. Results The in thyrocytes from GD and HT expression of Fas - FasL was higher than those in normal thyroid tissue. In GD and HT, Fas was positively related to FasL, and GD thyrocytes expressed Fas- FasL less than HT thyrocytes. Conclusion The expression of Fas and its ligand FasL is different in autoimmune thyroid disease (AITD), and GD thyrocytes expressed less than HT thyrocytes. The interaction between Fas and FasL on thyrocytes may represent their major mechanism of autoimmune depletion. Fas/FasL may contribute to the pathophysiology of autoimmune thyroid disease and play an important role in the pathogenesis of AITD.