| THE AUTOCRINE REGULATORY EFFECT OF VASOACTVE INTESTINAL PEPTIDE ON THE GROWTH OF HUMAN PANCREATIC CARCINOMA CELLS |
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| 引用本文： | 陈元方,陈潜,陆国钧,范振符,钟守先. THE AUTOCRINE REGULATORY EFFECT OF VASOACTVE INTESTINAL PEPTIDE ON THE GROWTH OF HUMAN PANCREATIC CARCINOMA CELLS[J]. 中国医学科学杂志(英文版), 1994, 0(4) |
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| 作者姓名： | 陈元方 陈潜 陆国钧 范振符 钟守先 |
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| 摘 要： | ＴＨＥＡＵＴＯＣＲＩＮＥＲＥＧＵＬＡＴＯＲＹＥＦＦＥＣＴＯＦＶＡＳＯＡＣＴＶＥＩＮＴＥＳＴＩＮＡＬＰＥＰＴＩＤＥＯＮＴＨＥＧＲＯＷＴＨＯＦＨＵＭＡＮＰＡＮＣＲＥＡＴＩＣＣＡＲＣＩＮＯＭＡＣＥＬＬＳ￥ＣｈｅｎＹｕａｎｆａｎｇ陈元方，ＣｈｅｎＱｉａｎ陈潜...
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THE AUTOCRINE REGULATORY EFFECT OF VASOACTVE INTESTINAL PEPTIDE ON THE GROWTH OF HUMAN PANCREATIC CARCINOMA CELLS |
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| Chen Yuanfang,Chen Qian,Lu Guojun,Fan Zhenfu and Zhong Shouxian. THE AUTOCRINE REGULATORY EFFECT OF VASOACTVE INTESTINAL PEPTIDE ON THE GROWTH OF HUMAN PANCREATIC CARCINOMA CELLS[J]. Chinese medical sciences journal, 1994, 0(4) |
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| Authors: | Chen Yuanfang Chen Qian Lu Guojun Fan Zhenfu Zhong Shouxian |
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| Abstract: | In the present study, the effects of VIP on the growth of two human pancreatic carcinoma cell lines PU-PAN-l and PANC-I were determined using tritiated thymidine incorporation. VIP receptors. intracellular cAMP and polyamines were investigated. The results indicated that VIP at a concentration of 10-8 mol/L to 10-7 mol/L can significantly stimulate the growth of PU-PAN-I cells but not PANC-1 cells. This effect is dose-dependent and abolished by VIP receptor antagonist, [4-C1-Phe6 . Leu17] VIP, suggesting VIP receptors in PU-PAN-I cells may mediate this effect. VIP can markedly elevate the levels of intracellular cAMP and polyamines in PU-PAN-I cells.indicating that the growth-promoting effect stimulated by VIP may be via a rapid increase in the biosyntheses of cAMP and polyamines. In addition, the VIP-antibody inhibited the growth of PU-PAN-I cells in serum-free culture mediurn. The results above suggested that VIP has an autocrine regulatory effect on this pancreatic carcinoma cell line (PU-PAN-1). |
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| Keywords: | vasoactive intestinal peptide(VIP) VIP receptors pancreatic carcinoma. |
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