调中颗粒对反流性食管炎大鼠食管黏膜脑肠肽和一氧化氮合酶的影响

目的: 研究调中颗粒对混合反流性食管炎大鼠食管黏膜血管活性肠肽(VIP)、P物质(SP)和一氧化氮合酶(NOS)水平的影响。 方法: 将大鼠随机分成5组(调中颗粒组、半夏泻心汤组、西沙必利组、空白模型组、假手术组),用食管十二指肠端侧吻合术法制备混合反流性食管炎大鼠模型,于给药12 d后处死大鼠,HE染色观察食道黏膜病理改变,免疫组织化学技术检测大鼠食管黏膜VIP,SP,NOS的水平。 结果: 与模型组比较,调中颗粒组、半夏泻心汤组、西沙必利组光镜下食管黏膜组织有不同程度的修复,食管黏膜VIP和NOS表达明显增强,而SP表达减弱;3个治疗组之间无明显差异。 结论: 调中颗粒可能通过使SP表达增强、VIP和NOS表达减少调节胃肠运动,从而抑制胃肠内容物的反流和促进黏膜的修复。

Influence of Tiaozhong Granule on Brain-gut Peptide and Nitricoxide Synthase of Rats with Mixed Reflux Esophagitis

Objective: To study the influence of the Tiaozhong Granule on the vasoactive intestinal peptide (VIP),substance P (SP)and nitricoxide synthase (NOS) of rats with mixed reflux esophagitis. Method: The rats were randomly divided into five groups:the model group,the normal group,the Tiaozhong granule Group,the Banxia Xiexin Tang group and cisapride group.Then anastomose esophagoduodenstom was used to make the rat model of mixed reflux esophagitis. The pathohistologic examination was used to observe the changes in esophagus mucous membrane.The content of SP,NOS and VIP in the esophagus tissue was detected after 12 days by immunohistochemical technique. Result: Compared with the model group, the esophagus mucous membrane of the Tiaozhong Granule group,the Banxia Xiexin Tang group and cisapride group were renovated;the content of NOS and VIP in the rats of the Tiaozhong granule group,the Banxia Xiexin Tang group and cisapride group were increased significantly;and the content of SP decreased after treatment.The differences between the treatment groups were not significant. Conclusion: Tiaozhong granule can be applied for mixed reflux esophagitis through decreasing the content of NOS,VIP and increasing SP in the esophagus tissue.