四君子汤对小鼠膀胱癌化疗的减毒增效作用

目的 探讨中药四君子汤对小鼠膀胱癌化疗的减毒增效作用。方法 采用小鼠膀胱癌细胞接种于T739小鼠皮下，动物随机分为8组：对照组，丝裂霉素C(mitomycin C，MMC)常规剂量组，MMC小剂量组，四君子汤大剂量组，四君子汤中剂量组，四君子汤小剂量组，联合用药(四君子汤大剂量+MMC常规剂量)A组，联合用药(四君子汤大剂量+MMC小剂量)B组；每组于接种癌细胞24h后分别给药；用药14天后分别检测各组小鼠的抑瘤率、腹腔巨噬细胞活性，观察小鼠荷瘤生存时间及治疗7天后血白细胞数的变化。结果 四君子汤大剂量组的荷瘤小鼠生存时间与对照组比较差异有显著性(P<0．05)，而四君子汤中、小剂量组与对照组比较差异无显著性(P>0．05)；MMC常规组的抑瘤率最高，但荷瘤生存时间与对照组比较差异无显著性(P>0．05)；联合用药B组的小鼠荷瘤生存时间最长，达到(32．7±1．3)天，与其他各组比较差异均有显著性(P<0．05)；联合用药B组除抑瘤率低于MMC常规组及联合用药A组，血白细胞数及巨噬细胞活性均优于MMC各剂量组及联合用药A组，差异均有显著性(P<0．05)。结论 四君子汤联合小剂量MMC化疗可以有效抑制小鼠膀胱肿瘤生长，具有增效减毒之作用。

Toxicity Attenuation and Efficacy Potentiation Effects of Sijunzi Decoction on Bladder Carcinoma Treated by Chemotherapy in Mice

OBJECTIVE: To investigate the toxicity attenuation and efficacy potentiation effects of Sijunzi decoction (SJZD) on bladder carcinoma treated by chemotherapy in mice. METHODS: T739 mice were randomly divided into 8 groups after subcutaneous inoculation of bladder carcinoma cells, the control group (A); two mitomycin C (MMC) group, treated with MMC of routine dosage (B) and low-dosage (C) respectively; three SJZD groups, treated with SJZD of high (D), medium (E) and low-dosage (F) respectively; and two combined treatment groups, treated with SJZD of high-dosage + MMC of routine dosage(G) and SJZD of high-dosage + MMC of low-dosage(H). The medication was begun at 24 hrs after inoculation. The tumor inhibitory rate, activity of peritoneal macrophages after 14 days of treatment and change of peripheral white blood cells after 7 days of treatment were determined and the survival time of mice was observed. RESULTS: The survival time of mice in Group D was significantly higher than that in Group A (P < 0.05), while those in Group E and F showed insignificant difference as compared with those in Group A (P > 0.05). The highest tumor inhibitory rate was shown in Group B, but the survival time in that group showed no significant difference as compared to those in Group A (P > 0.05). The longest survival time (32.7 +/- 1.3 days) was shown in Group H, which was obviously different to that in other groups (P < 0.05). And the leukocyte counts and macrophage activity in Group H were better than those in Group B, C and G (P < 0.05), except that the tumor inhibitory rate was significantly lower than that in Group B, C and G (P < 0.05). CONCLUSION: Combined chemotherapy of SJZD with low dosage MMC has definite effect in inhibiting tumor growth in mice with bladder carcinoma, displaying special effects of toxicity attenuation and efficacy potentiation.