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| 丙型肝炎病毒非结构蛋白2反式调节基因NS2TP的克隆化 | |
| 引用本文： | 张黎颖,成军,邓红,刘妍,王琳. 丙型肝炎病毒非结构蛋白2反式调节基因NS2TP的克隆化[J]. 世界华人消化杂志, 2005, 13(14): 1700-1704 |
| 作者姓名： | 张黎颖 成军 邓红 刘妍 王琳 |
| 作者单位： | 1. 北京地坛医院传染病研究所,北京市,100011 2. 西安交通大学第二医院感染科,陕西省,西安市,710004 3. 中国人民解放军第302医院传染病研究所基因治疗研究中心,北京市,100039 |
| 基金项目： | 国家自然科学基金攻关项目,No．C03011402,No．C30070689军队“九、五”科技攻关项目,No．98D063军队回国留学人员启动基金项目,No．98H038军队“十、五”科技攻关青年基金项目,No．01Q138军队“十、五”科技攻关项目,No．01MB135~~ |
| 摘 要： | |
| 关 键 词： | 丙型肝炎病毒 非结构蛋白2 反式调节 克隆 |
| 修稿时间： | 2005-01-11 |
Cloning and identification of gene NS2TP transregulated by non-structural protein 2 of hepatitis C virus |
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| Li-Ying Zhang,Jun Cheng,Hong Deng,Yan Liu,Lin Wang. Cloning and identification of gene NS2TP transregulated by non-structural protein 2 of hepatitis C virus[J]. World Chinese Journal of Digestology, 2005, 13(14): 1700-1704 | |
| Authors: | Li-Ying Zhang Jun Cheng Hong Deng Yan Liu Lin Wang |
| Affiliation: | Li-Ying Zhang,Jun Cheng,Institute of Infectious Diseases,Beijing Ditan Hospital,Beijing 100011,China Hong Deng,Department of Infectious Diseases,the Second Hospital of Xi'an Jiaotong University Xi'an 710004,China Yan Liu,Lin Wang,Research Center of Gene Therapy,the 302 Hospital of Chinese PLA,Beijing 100039,China |
| Abstract: | AIM: To clone and identify the new gene NS2TP transregulated by the non-structural protein 2 of hepatitis C virus. METHODS: Based on the subtractive cDNA library of genes transregulated by NS2 protein of hepatitis C virus, the coding sequence of the new gene was obtained by bioinformatics methods. Polymerase chain reaction (PCR) was conducted to amplify NS2TP gene. RESULTS: The coding sequence of new gene was cloned and identified successfully. The coding region of NS2TP gene had a length of 456 nucleotides and the coding product pocessed 151 amino acid residues. After searching in GenBank and SwissProt, the new gene had no significant homology with the genes we have known, and its functions remained unknown. CONCLUSION: A new target gene, transregulated by HCV NS2 protein, is recognized. |
| Keywords: | Hepatitis C virus Non-structural protein 2 Transregulation Clone |
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