苏黄止咳胶囊改善哮喘豚鼠气道重塑的作用及其机制

目的 探讨苏黄止咳胶囊改善哮喘豚鼠气道重塑的作用及其机制.方法 36只豚鼠随机分为正常组,模型组,苏黄止咳胶囊低、中、高剂量组(1.5、3、6g/kg),阳性对照组(沙丁胺醇,5 mg/kg).除正常组外,各组豚鼠均腹腔注射1 mg/mL卵清白蛋白(OVA)和10 mg/mL Al (OH)3混悬液1 mL,同时以1％...

Effects and mechanism of Suhuang Antitussive Capsules in relieving airway remodeling of asthmatic guinea pigs

AIM To investigate the effects and mechanism of Suhuang Antitussive Capsules in relieving airway remodeling of asthmatic guinea pigs.METHODS Thirty-six guinea pigs were randomly divided into blank group,model group,Suhuang Antitussive Capsules groups(1.5 g/kg low-dose,3 g/kg medium-dose,6 g/kg high-dose),and salbutamol group(5 mg/kg).Except for the blank group,the other groups had their guinea pigs induced into asthma models given 2-week consecutive administration of intraperitoneal injection of a mixture solution of 1 mL 1 mg/mL OVA and 10 mg/mL Al(OH)3,and 1%OVA stimulation every other day.At 1 h after the last administration,the guinea pigs were subjected to the detection of their cough latency;the pathological observation of the lung and trachea tissues by HE staining;the measurement of the levels of inflammatory factors IL-4,IL-5 and histamine by ELISA;and determination of several related proteins expressions in the lung tissue by immunoblot;the verification of the effects of Suhuang Antitussive Capsules on bronchodiastolic rate in guinea pigs;the identification of in vitro effects of Suhuang Antitussive Capsules(125,250,500μg/mL)and aminophylline(50μg/mL)on proliferation activity in histamine-induced RBSMCs by MTT,the capsules’effect on PCNA expressions by immunofluorescence,and on the expression of JAK2,STAT3,Akt and cell cycle-related proteins by immunoblot.RESULTS Compared with the model group,groups intervened with Suhuang Antitussive Capsules shared significantly prolonged cough latency,effectively relieved thickening of airway smooth muscle in trachea tissues,improved inflammation of lung tissue,and reduced levels of inflammatory factors IL-4,IL-5 and histamine in lung tissues;significantly improved bronchodiastolic rate in isolated guinea pigs.In contrast to the model group,Suhuang Antitussive Capsules groups displayed significantly inhibited RBSMCs proliferation,ameliorated expressions of PCNA,JAK2,STAT3,Akt and cell cycle-related proteins.CONCLUSION Suhuang Antitussive Capsules alleviate the asthma in guinea pigs via inhibiting RBSMCs proliferation,improving bronchial smooth muscle hyperplasia and airway remodeling,and regulating cell cycle-related proteins through JAK/STAT signal pathway.