基于网络药理学的“葛花-枳椇子”药对治疗酒精性肝病作用机制研究＊

目的 采用网络药理学方法分析“葛花-枳椇子”药对治疗酒精性肝病的药物成分、作用靶点、主要通路及生物过程,探讨其药效物质基础和配伍机制。方法 依托中药系统药理学分析平台（TCMSP）、中医药整合药理学研究平台v2.0（TCMIP）及Drugbank数据库检索葛花、枳椇子相关的化学成分和作用靶点,在UniProt 蛋白数据库检索对应的人类蛋白基因,通过NCBI获取与酒精性肝病相关的疾病基因靶点信息,运用STRING平台构建PPI网络,并在Cytoscape中利用BINGO插件对靶点基因进行GO生物过程富集分析,利用DAVID生物学信息数据库对靶点进行KEGG信号通路分析,并绘制分子机制图。结果 本研究共获得21种化合物成分,这些成分靶点与酒精性肝病共有6个基因靶点、14条主要信号通路。参与的基因有ALDH2、IL6、TNF、HMOX1、HSPA5和MTTP;“葛花-枳椇子”药对治疗酒精性肝病主要参与Toll样受体信号（TLR）通路和NOD样受体信号（NLR）通路。结论 本研究初步验证了“葛花-枳椇子”药对治疗酒精性肝病的基本药理作用及相关机制,为进一步研究其作用机制奠定了基础。

Research on the Mechanism of Flos Puerariae-Semen Hoveniae Herbal Pair in the Treatment of Alcoholic Liver Disease Based on Network Pharmacology

Objective To investigate the efficacy and compatibility mechanism of Flos Puerariae-Semen Hoveniae (GH-ZJZ) herbal pair in the treatment of alcoholic liver disease (ALD) based on network pharmacology.Methods All of the chemical compounds related to GH-ZJZ herbal pair were researched from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP), Integrative Pharmacology-based Research Platform of Traditional Chinese Medicine (TCMIP v2.0) and the Drugbank database, to explore potential targets related to candidate compounds. Gene''s target information related to ALD was searched through the national biological information center (NCBI). The compound-target-disease network was constructed through network pharmacology. Protein-to-protein interaction (PPI) data were downloaded from STRING and then PPI core genes were constructed. Gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis of key target genes were performed. The molecular mechanism and compound-target-disease network diagram were drawn.Results A total of 19 active components were obtained, which acted on 6 targets and 14 major pathways respectively. The genes involved were ALDH2, IL6, TNF, HMOX1, HSPA5, and MTTP; Toll-like receptor signaling (TLR) and NOD-like receptor signaling (NLR) were mainly involved in the GH-ZJZ herbal pair in treatment of ALD.Conclusion The result of the study preliminarily verifies the basic pharmacological effects and related mechanisms of GH-ZJZ herbal pair in the treatment of ALD, and lays a foundation for further studies on the mechanism of action.