金葡菌对U937细胞XIAP和cIAP1/2表达的影响

目的探讨X 相关凋亡抑制蛋白（XIAP）和cIAP1/2在金黄色葡萄球菌（简称金葡菌）诱导的人巨噬细胞系U937细胞凋亡中的作用。方法采用Western 印迹分析检测金葡菌感染不同时间后XIAP和cIAP1/2的表达水平；预先用不同浓度的Embelin（XIAP抑制剂）处理U937细胞60 min，观察金葡菌感染30 min后U937细胞的凋亡情况。结果随着感染时间的延长，XIAP和cIAP1/2的表达逐渐下降。加入XIAP的抑制剂Embelin后，U937细胞的凋亡率随着Embelin的浓度增加而逐渐升高。结论XIAP和cIAP1/2参与了金葡菌诱导的U937细胞凋亡过程。Embelin通过特异性地抑制XIAP表达增加金葡菌诱导的U937细胞凋亡率。

Roles of XIAP1/2 and cIAP in the apoptosis of U937 cell lines induced by Staphylococcus aureus

The aim of this study was to investigate the role of X-linked inhibitor of apoptosis protein（XIAP）and cIAP1/2 in the apoptosis of human monocytic U937 cells induced by Staphylococcus aureus（S.aureus）.U937 cells were treated with S.aureus at different time points,and Western blot were performed to detect the expressions of XIAP and cIAP1/2.The U937 cells were pretreated with Embelin（an inhibitor for XIAP）,and the apoptosis rates of U937 cells were detected by Annexin V FITC/PI assay.The results showed that the downregulation of XIAP and cIAP1/2 by S.aureus were statistically significant and inhibition of XIAP with Embelin caused further increase in apoptosis of U937 cells.It is concluded that XIAP and cIAP1/2 participated in the apoptosis of U937 cells.Embelin increases U937 cells apoptosis induced by S.aureus via inhibiting the expression of XIAP.