体外培养人脐带血间充质干细胞向胰岛细胞分化及对糖尿病治疗的实验研究

目的研究人脐带血间充质干细胞向胰岛素分泌细胞分化的潜能及其移植后对糖尿病大鼠的治疗效果。方法体外分离培养HUCB-MSCs,在胰岛细胞培养条件下经药物定向诱导其分化;免疫组化对诱导细胞进行胰岛β细胞标记鉴定;双硫腙染色鉴定锌离子表达及检测胰岛样细胞的移植效果。结果 HUCB-MSCs经诱导后,免疫细胞化学染色显示表达人胰岛素;双硫腙染色呈棕红色;移植后2周,胰岛样细胞组血糖浓度明显降低。结论 HUCB-MSCs在体外诱导培养条件下,具有向胰岛素分泌细胞分化的潜能,这种细胞可能为Ⅰ型糖尿病提供一条新的治疗途径。

Inducing human umbilical cord blood mesenchymal stem cells into insulin secreting cells in vitro culture and its effects on diabetes rats

Objective To explore the possibility of inducing the differentiation of human umbilical cord blood mesenchymal stem cells（HUCB-MSCs） into insulin secreting ceils in vitro and the therapeutic efficacy on diabetic rats following transplantation. Methods We isolated and cultured HUCB-MSCs, and induced HUCB- MSCs to differentiate into insulin secreting cells in the islet cell culture condition. HUCB-MSCs of induction were detected by immunocytochemical Methods. Zinchydronium was detected by Dithizon staining. Transplantation efficiency of islet-like ceils was detected. Results Under induction, immunocytochemical examination showed that the expression of human insulin was positive. Dithizon stain showed that the cytoplasm of HUCB-MSCs was stained in brownish red color after induction. At 2 weeks following transplantation, blood glucose concentration in the islet-like cell group significantly reduced. Conclusion HUCB-MSCs can be differentiated into insulin secreting ceils in vitro. HUCB-MSCs have the potential to become an excellent candidate in β cell replacement therapy of type Ⅰ diabetes.