高效液相色谱法测定合剂中苯甲酸的含量 |
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引用本文: | 陈媛 丘增萍 吴楣 等. 高效液相色谱法测定合剂中苯甲酸的含量[J]. 中国现代药物应用, 2014, 0(11): 1-3 |
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作者姓名: | 陈媛 丘增萍 吴楣 等 |
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作者单位: | [1]梅州市食品药品监督检验所,514071; [2]嘉应学院医学院,514071; |
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摘 要: | 目的:采用高效液相色谱法(HPLC法)测定合剂(前胡桑菊合剂、五柑合剂、胃肠合剂、佛耳合剂)中苯甲酸的含量。方法用乙酸乙酯提取合剂中的苯甲酸,提取液浓缩后采用HPLC法进行测定。选择Thermo Syncronis C18(250 mm×4.6 mm,5μm),以甲醇-磷酸二氢钾(0.05 mol/L)(45:55)为流动相,流速为1.0 ml/min,柱温为30℃,检测波长为226 nm。结果苯甲酸回归方程Y=5835283.5514 X-23440.3860(R=0.9999),在0.020~0.1018 mg/ml范围内线性关系良好;前胡桑菊合剂、五柑合剂、胃肠合剂、佛耳合剂平均回收率分别为99.79%、99.37%、99.34%、99.92%;RSD分别为0.35%、0.10%、0.76%、0.36%(n=3)。结论测定方法简便、准确、专属性强、重现性良好。
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关 键 词: | 高效液相色谱 合剂 苯甲酸 含量测定 |
Determination of benzoic acid in mixture by HPLC |
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Affiliation: | CHEN Yuan, QIU Zeng-ping, WU Mei, et al. (Mei zhou Institute for Food and Drug Control, Meizhou 514071, China) |
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Abstract: | Objective To establish a high performance liquid chromatography(HPLC) method for the determination of(Qianhu Sangju mixture, Wugan mixture, Weichang mixture, Foer mixture) benzoic acid. Methods The extraction mixture of benzoic acid with ethyl acetate, the concentrated extract was determined by HPLC method. Select the thermo syncronis C-18(250 mm×4.6 mm, 5 μm), with methanol and potassium dihydrogen phosphate(0.05 mol/L) (45:55) as mobile phase, flow rate of 1.0 ml/min, column temperature was 30℃ , the detection wavelength was 226 nm. Results Benzoic acid regression equation was Y=5835283.5514 X-23440.3860(R=0.9999), in 0.020~0.1018 mg/ml the linear relationship was good; Qinhu Sangju mixture, Wugan mixture, Weichang mixture, Foer mixture the average recovery rate was 99.79%, 99.37%, 99.34%, 99.92%;RSD=0.35%, 0.10%, 0.76%, 0.36%(n=3). Conclusion This method is simple and accurate, with a strong specificity, so it can be used as preparation for the quality control. |
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Keywords: | High performance liquid chromatography Mixture Benzoic acid Content determination |
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