| 一阶导数紫外光谱法测定辅酶Q_(10)胶囊的含量 |
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| 引用本文： | 方兴华!314001,徐宏祥!314001. 一阶导数紫外光谱法测定辅酶Q_(10)胶囊的含量[J]. 中国生化药物杂志, 2000, 0(3) |
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| 作者姓名： | 方兴华!314001 徐宏祥!314001 |
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| 作者单位： | 浙江省嘉兴市药品检验所 |
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| 摘 要： | 目的 :建立用一阶导数紫外光谱法快速测定辅酶Q10 胶囊含量的方法。方法 :用无水乙醇作溶剂 ,以一阶导数紫外光谱在 2 5 6～ 2 81nm峰 谷间距离D作为定量依据 ,用标准对照法计算含量。结果 :辅酶Q10 浓度在5～ 40 μg/ml之间与一阶导数紫外光谱在 2 5 6～ 2 81nm峰 谷间距离D呈良好线性关系 (r=0 .9999) ,平均回收率为 1 0 1 .1 % ,RSD为 1 .2 % (n =5 )。结论 :此法简便、准确 ,重现性好。
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| 关 键 词： | 辅酶Q_(10)胶囊 一阶导数紫外光谱法 含量测定 |
Quantitative Determination of Coenzyme Q_(10) Capsules by the First Derivative Spectrophotometry |
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| Fang Xinghua,Xu Hongxiang Jiaxing Institute for Drug Control of Zhejiang Province. Quantitative Determination of Coenzyme Q_(10) Capsules by the First Derivative Spectrophotometry[J]. Chinese Journal of Biochemical Pharmaceutics, 2000, 0(3) |
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| Authors: | Fang Xinghua Xu Hongxiang Jiaxing Institute for Drug Control of Zhejiang Province |
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| Affiliation: | Jiaxing 314001 |
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| Abstract: | Purpose:A new simple method for rapid determination of Coenzyme Q_(10) in capsules was established by the first derivative spectrophotometry. Methods:Anhydrous ethanol was used as solvent and the assay was calculated by the distance D between the first derivative peak at 256nm and valley at 281nm through comparing sample with standard.Results:There was a linear relationship between Coenzyme Q 10 concentration in the range of 5～40μg/ml with the distance Dof first derivative peak valley at 256～281nm (r=0.999 9), the average recovery rate was 101.1%, and the relative standard deviation was 1.2% (n=5). Conclusion:The proposed method is simple, accurate and reproducible. |
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| Keywords: | Coenzyme Q_(10) capsules First derivative spectrophotometry Assay |
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