Characterization of a novel allelic variant in HLA‐B*40 lineage,HLA‐B*40:298:02, by cloning and sequencing |
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| Authors: | W Tian F M Zhu W Y Wang L X Li J H Cai |
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| Institution: | 1. Immunogenetics Research Group, Department of Immunology, College of Basic Medical Sciences, Central South University, Changsha, China;2. HLA Typing Laboratory, Blood Center of Zhejiang Province, Hangzhou, Zhejiang, China |
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| Abstract: | A novel allelic variant in HLA‐B*40 lineage, HLA‐B*40:298:02, has been identified in an individual of Han ethnicity afflicted with nasopharyngeal carcinoma in Hunan province, southern China. Following polymerase chain reaction–Sanger sequence‐based typing (PCR–SBT), this new variant was further confirmed by two distinct strategies of cloning and sequencing. HLA‐B*40:298:02 differs from HLA‐B*40:298:01 by a single synonymous cytosine substitution at nucleotide position 26 (T→C) in exon 3, which corresponds to codon 99 of the mature HLA‐B mRNA molecule. This new allele has an estimated frequency of 0.0002, in about 2,500 sequence‐based typed subjects from the same population. |
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| Keywords: | cloning HLA‐B*40:298:02 long‐range PCR sequence‐based typing southern Chinese Han population |
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