伊达拉奉对沙土鼠全脑缺血再灌注损伤后海马CAI区神经元凋亡相关基因Bcl-2、Bax表达的影响

目的 :探讨伊达拉奉对沙土鼠全脑缺血再灌注损伤后海马CAI区神经元凋亡相关基因Bcl 2、Bax表达的影响及可能的作用机制。方法 :采用Global模型造成沙土鼠全脑缺血损伤模型 ;腹腔注射伊达拉奉对其进行治疗后运用免疫组织化学方法 ,检测受损脑组织海马CAI区神经元中的Bcl 2、Bax免疫反映阳性细胞数量。结果 :治疗组大鼠脑组织中Bcl 2表达明显增加 ,Bax表达明显低于损伤对照组 (P <0 0 5 )。结论 :伊达拉奉通过清除羟自由基 ,抗脂质过氧化 ;可能通过增强Bcl 2的表达 ,抑制Bax的表达来减少脑缺血后神经细胞的凋亡 ,发挥脑保护作用。

Study on Effect of Edaravone on Expression of Apoptosis-related gene:Bcl-2,Bax in Hippocampal CAI Region after Cerebral Ischemia/Reperfusion in Rats

s] Objective: To discuss the effect and possible me ch anism of Edaravone on expression of apoptosis-related gene: Bcl-2, Bax in hippocampal CAI region after cerebral ischemia in gerbils. Methods:Gerbils were treated with Edaravone injection into abdominal cavity after cere bral ischemia/reperfusion(I/R) and then immunohistochemistry was used to detec t the expression of Bcl-2,Bax in neuron of hippocampal CAI region. R esults: Compared with the control group,the expression of Bcl-2 in hippocampal CAI region increased obviously while the expression of Bax decreased in treated group. Conclusion:Edaravone may red uce the apoptosis of neuron after I/R through enhancing the expression of Bcl -2 and inhibiting the expression of Bax.But its detailed mechanism is sti ll in need for further research. [