柚皮苷-壳聚糖/羟基磷灰石复合支架修复大鼠颅骨缺损

背景:骨碎补的有效成分柚皮苷具有补肝肾强筋骨的传统功效,能增加骨痂厚度,提高骨折愈合质量。目的:探究载中药骨碎补有效成分柚皮苷壳聚糖/羟基磷灰石复合支架的骨传导和骨诱导性能。方法:将一定钙磷比的羟基磷灰石前体液与含柚皮苷的壳聚糖溶液在碱性条件下原位结晶、冷冻干燥,获得柚皮苷-壳聚糖/羟基磷灰石多孔支架。将15只成年SD大鼠随机分成空白组(n=5)、对照组(n=5)和实验组(n=5),建立直径5 mm颅骨骨缺损模型,空白组未填充生物材料,对照组填充壳聚糖/羟基磷灰石支架,实验组填充柚皮苷-壳聚糖/羟基磷灰石复合支架。术后4周取材,CT扫描观察颅骨修复情况,苏木精-伊红染色观察颅骨修复的形态学,骨形态发生蛋白2和血管内皮生长因子免疫组化染色后观察缺损区域局部成骨活性因子的表达。结果与结论:(1)CT扫描显示,空白组大鼠颅骨未见明显骨生成,仅在缺损边缘可见少量新生骨;对照组于缺损孔隙可见新生骨形成,新生骨较少;实验组骨缺损修复良好,新生骨组织与缺损孔隙周围颅骨密度相似,大面积新生骨广泛填充了缺损孔隙。(2)苏木精-伊红染色显示,空白组缺损区填充以稀薄的疏松网状纤维组织,可见大量炎性反应病灶,...

Naringin-chitosan/hydroxyapatite composite scaffold in repair of rat skull defect

BACKGROUND: Naringin, the active ingredient of Rhizoma Drynariae, has the traditional effect of strengthening the liver and kidney and strengthening the bones and muscles, increasing the thickness of the callus and improving the quality of fracture healing. OBJECTIVE: To explore the bone conduction and bone induction properties of naringin-chitosan/hydroxyapatite composite scaffolds. METHODS: The hydroxyapatite precursor solution with a certain calcium to phosphorus ratio and the chitosan solution containing naringin were crystallized in situ under alkaline conditions and freeze-dried to obtain a naringin-chitosan/hydroxyapatite porous scaffold. A total of 15 SD rats were randomly divided into blank group (n=5), control group (n=5) and experimental group (n=5). The 5 mm-diameter skull bone defect models were established by drilling holes. Biomaterials were not filled in the blank group. Chitosan/hydroxyapatite scaffolds were filled in the control group, and naringin-chitosan/hydroxyapatite scaffolds were filled in the experimental group. At 4 weeks after surgery, CT scans were performed to observe the skull repair. Hematoxylin-eosin staining was used to observe morphological differences. Immunohistochemical staining of bone morphogenetic protein 2 and vascular endothelial growth factor was performed to observe the expression of local osteogenic active factors in the defect areas. RESULTS AND CONCLUSION: (1) CT scan showed that no obvious osteogenesis was seen in the skull of rats in the blank group, only a small amount of new bone was seen at the edge of the defect. In the control group, new bone formation could be seen in the defect pores, and there was less new bone. In the experimental group, the bone defect was well repaired, the density of new bone tissue and the skull around the defect pores were similar, and a large area of new bone widely filled the defect pores. (2) Hematoxylin-eosin staining showed that the defect area in the blank group was filled with thin loose reticular fibrous tissue, and a large number of inflammatory response lesions were seen, and only a small amount of new bone was formed at the edge of the defect. In the control group and the experimental group, residual scaffold material, new bone trabecula and osteoblasts were seen in the defect area, osteoblasts were distributed in clusters in the pores of the scaffold and the edges of the defect area, surrounded by a large number of capillaries. Among them, the experimental group showed a stronger ability of new bone growth. (3) Immunohistochemical staining showed that the expression levels of local osteogenic activity factors bone morphogenetic protein 2 and vascular endothelial growth factor in the experimental group were higher than those in the control and blank groups (P < 0.05). (4) The results conclude that naringin-chitosan/hydroxyapatite composite scaffolds can provide necessary carrier for bone defect repair. Naringin can create local osteogenic microenvironment, accelerate the growth and mineralization of new bone tissue, and have good bone repair performance. © 2022, Publishing House of Chinese Journal of Tissue Engineering Research. All rights reserved.