Circ_0061140 stimulates the malignant development of prostate cancer by targeting miR-1193 |
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Authors: | Kai Wang Yi Fan Ji Sun Liwei Zhao Yufu Yu Gonghui Li |
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Institution: | 1.Department of Urology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China;2.Department of Urology, Zhejiang Xiaoshan Hospital Affiliated to Hangzhou Normal University, Hangzhou, China |
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Abstract: | BackgroundThis study sought to explore the expression pattern in prostate cancer (PCa) tissues, as well as the regulatory effects of circ_0061140 on the proliferative potential of PCa cells.MethodsA quantitative real-time polymerase chain reaction (qRT-PCR) analysis was undertaken to detect circ_0061140 levels in 43 paired PCa tissues and adjacent normal tissues. After the knockdown of circ_0061140, changes in the proliferative potential of PCa cells and tumor growth in nude mice with PCa were detected. Finally, the relationship of circ_0061140 and miR-1193 in the development of PCa was assessed.ResultsThe results showed that circ_0061140 was upregulated in PCa tissues. PCa patients with higher Gleason score or larger sized tumors expressed higher levels of circ_0061140. Additionally, the knockdown of circ_0061140 inhibited the proliferative potential of PCa cells. MiR-1193 was the target gene binding circ_0061140, and its level was negatively regulated by circ_0061140. Finally, rescue experiments showed that miR-1193 was regulated by circ_0061140 in the development of PCa.ConclusionsCirc_0061140 is upregulated in PCa tissues, and is closely linked to Gleason score and tumor size in PCa. Additionally, it causes PCa cells to proliferate by negatively regulating miR-1193. |
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Keywords: | Circ_0061140 miR-1193 prostate cancer (PCa) malignant development |
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