CD30在肾综合征出血热患者T细胞表达的检测及其临床意义

目的 :研究CD3 0 在肾综合征出血热 (HFRS)患者急性期外周血T淋巴细胞亚群的表达及意义。方法 :应用免疫荧光抗体双重标记和流式细胞仪技术分析CD3 0 在HFRS患者急性期外周血CD 4,CD 8T细胞表达的水平。结果 :CD 4CD-3 0 细胞在重型组 ,中轻型组和正常组三组间均无显著差异 (P >0 0 5 ) ;患者CD 4CD 3 0 ,CD 8CD-3 0 细胞明显增加 ,且重型组 ,中轻型组和正常组三组间均有极显著差异 (P <0 0 1)。结论 :HFRS患者急性期存在体液免疫和细胞免疫功能亢进 ,各种T淋巴细胞亚群的比例失衡与该病免疫发病机制及病情的严重程度密切相关。

The determination of CD30 expression on peripheral blood T lymphocyte subsets in patients with hemorrhagic fever with renal syndrome by FCM

Objective:To determine the CD 30 expression in patients with hemorrhagic fever with renal syndrome(HFRS).Methods:The double immunofluorescence technique and flow cytometry were used.Results:There is no significant difference among the three groups of HFRS patients on the CD 4CD - 30 T lymphocyte subset.While the CD 4CD 30 T cells increase and the difference between severe group and mild-moderate group is very significant(P<0.01);that between the mild-moderate group and normal control is significant(P<0.05).The CD 8CD - 30 T cells increase while the CD 8CD 30 T cells decrease obviously in HFRS patients,and the differences among three groups on both subsets are significant(P<0.01).Conclusion:The humoral immune and cytoimmune are synthenic in HFRS patients during acute phase.The loss of balance between T lymphocytes may play an important role in the pathophysiology of HFRS and is closely correlated to the severity of the HFRS.