Cloning and characterisation of the ribosomal RNA genes of the dimorphic yeast,Yarrowia lipolytica |
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| Authors: | Jeffrey J. Clare Lance S. Davidow David C. J. Gardner Stephen G. Oliver |
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| Affiliation: | (1) Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute of Science and Technology, P. O. Box 88, M60 1QD Manchester, UK;(2) Pfizer Central Research, Molecular Genetics Division, 06340 Groton, CT, USA;(3) Present address: Department of Microbiology, University of Connecticut, Health Center, 06032 Farmington, CT, USA |
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| Abstract: | Summary The ribosomal RNA genes of Yarrowia lipolytica have been identified, both in restriction digests of total genomic DNA and in a pBR322 gene bank, by hybridisation with cloned Saccharomyces cerevisiae rDNA. The Y. lipolytica rDNA repeat unit is 8.9 kb in size and contains the genes for the 25S and 18S, but not the 5S, rRNA species. The number of copies of these repeat units is approx. 50 per haploid genome. Several clones were found which did not conform to the standard restriction map due to differences outside the coding region. It appears that there is either heterogeneity of the spacer sequence within a strain or that the Y. lipolytica rDNA genes may be present as a number of separate clusters within this yeast's genome. |
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| Keywords: | rRNA genes Yeast Yarrowia lipolytica |
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