骨康方君臣佐使药治疗骨质疏松药理机制探讨

目的 基于网络药理学分析骨康方中药治疗骨质疏松(OP)的具体作用机制及其作用靶点、通路的异同.方法 首先通过数据库以及文献检索分别筛选出骨康方中君臣佐使各药的主要活性成分及其作用靶点;同时利用网络数据库得到OP相关的靶点;通过Venny 2.1分别筛选出君臣佐使-OP的交集靶点;然后通过STRING和Cytoscape...

Study on the pharmacological mechanism of the monarch, minister, assistant, and guide of Gukang decoction in the treatment of osteoporosis

Objective Based on network pharmacology, this paper analyzes the specific mechanism of monarch, minister, assistant, and guide of Gukang decoction in the treatment of osteoporosis (OP), and discusses the similarities and differences of its target and pathway. Method First, the main active components and related targets of monarch, minister, assistant, and guide of Gukang decoction were screened through database and literature search. At the same time, the OP-related targets were obtained from the network database. The intersection between targets of monarch, minister, assistant and guide-OP was screened with Venny 2.1. Protein-protein interaction (PPI) networks of intersection targets were constructed with STRING and Cytoscape software, respectively. Finally, the KEGG pathway of key targets was analyzed using DAVID database, and the KEGG results were interleaved with Venny 2.1. Result The key targets of monarch, minister, assistant, and guide - OP were obtained by screening and intersection, including 6 monarch drugs, 30 minister drugs, 31 assistant drugs, and 30 guide drugs. The enrichment results of KEGG pathway showed that there were 9 common pathways of key targets of monarch, minister, assistant, and guide, 7 specific pathways of monarch drugs, 2 specific pathways of minister drugs, and 1 specific pathway of guide drugs, but no specific pathway of assistant drugs. Conclusion Monarch, minister, assistant, and guide of Gukang decoction have common regulation pathway, which may treat OP through MAPK pathway. At the same time, they have their own special regulatory mechanism and play a unique role.