葛根素介导PTEN-PI3K-AKT信号通路抑制绝经后骨质疏松症的机制研究

目的 观察葛根素对去卵巢骨质疏松症小鼠骨组织形态的影响以及PTEN-PI3K-AKT信号通路的变化情况，并探讨其分子机制。方法 选取10～12周龄，体重约20～25 g的C57BL/6雌性小鼠24只，随机分为假手术组(Sham)、去卵巢组(Ovx)、葛根素组(Pue)。术后8周，鉴定为骨质疏松模型后开始给药干预，连续给药12周，每组随机取6只小鼠采取颈椎脱臼法处死。取左侧股骨进行Micro-CT扫描并通过real-time PCR法检测骨组织内PTEN、Foxo1、catalase基因的相对表达量。取右侧股骨制成石蜡切片观察其形态结构并采用免疫组化方法观察骨组织中PTEN、P-AKT、catalase蛋白的表达情况。结果术后8周Ovx组，小鼠子宫萎缩变小、骨组织HE染色骨小梁明显稀疏、Micro-CT骨小梁断裂明显。给药后，Pue组骨小梁增多、骨髓腔缩小，骨连接增多; PCR结果表明，葛根素干预后PTEN、Foxo1、catalase基因表达上调(P<0.05，差异有统计学意义);免疫组化结果表明，PTEN、catalase蛋白表达上调，P-AKT蛋白下调，但变化差异不明显。结论...

The mechanism study of inhibition effect of puerarin on osteoporosis through PTEN-PI3K-AKT signaling pathway

Objective To observe the effect of puerarin on bone morphology and PTEN-PI3K-AKT signaling pathway in ovariectomized osteoporosis mice, and to explore its possible mechanism. Methods Twenty-four C57BL/6 female mice aged 10-12 weeks and weighed 20-25g were randomly divided into sham operation group (sham), ovariectomy group (OVX), and puerarin group (PUE). At 8 weeks after operation, the intervention was given for 8 consecutive weeks. Six mice from each group were randomly selected and killed by cervical dislocation. The left femur was scanned with micro CT. The relative gene expression of PTEN, FoxO1, and catalase in the bone tissue was detected using real-time PCR. The protein expression of PTEN, p-Akt, and catalase in bone tissue was observed with immunohistochemistry. Results At eight weeks after operation, the uterus in OVX group became smaller, HE staining of the bone tissue was significantly sparse, and the trabecular fracture was obvious in micro CT. The results of PCR showed that the gene expression of PTEN, FoxO1, and catalase was up-regulated. The protein expression of PTEN and catalase was up-regulated, and the protein expression of p-Akt was down regulated in puerarin group, but the difference was not obvious. Conclusion Puerarin may inhibit postmenopausal osteoporosis by mediating PTEN-PI3K-AKT signaling pathway.