| 康莱特注射液诱发肾癌细胞凋亡及p53,bcl—2表达的研究 |
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| 引用本文： | 王俊杰 孙新臣. 康莱特注射液诱发肾癌细胞凋亡及p53,bcl—2表达的研究[J]. 中国肿瘤临床, 1999, 26(6): 439-442 |
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| 作者姓名： | 王俊杰 孙新臣 |
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| 作者单位： | 北京医科大学第一医院肿瘤治疗中心，北京医科大学第一医院泌尿外科研究所 |
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| 摘 要： | 目的；探讨康莱特注射液抗肿瘤的作用机制。方法：利用ＭＴＴ法分析康莱特肾癌细胞的抑制作用，末端脱氧核苷酰转移酶法检测细胞凋亡，免疫组织化学法分析ｐ５３和ｂｃｌ－２基因表达的影响。结果：康莱特抑制肾癌细胞的ＩＣ５０为１９．３１μｌ／ｍｌ，５μｌ／ｍｌ，和１０μｌ／ｍｌ康莱特注射液具有诱发细胞凋亡的作用，细胞凋亡分别为３１．３０％和８９．７６％，康莱特浓度继续增加时，细胞凋亡的数量反而减少，１５μdispla
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| 关 键 词： | 康莱特注射液 细胞凋亡 p53 bcl－2 肾癌细胞 |
Apoptosis Induced by Kang-Lai-Te Injection and Its Relation withExpression of p53, bcl-2 in Renal Cancer Cell Lines |
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| Wang Junjie Sun Xinchen Shen Wenjiang et al Cancer Therapy Center of Beijing Medical University,Beijing. Apoptosis Induced by Kang-Lai-Te Injection and Its Relation withExpression of p53, bcl-2 in Renal Cancer Cell Lines[J]. Chinese Journal of Clinical Oncology, 1999, 26(6): 439-442 |
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| Authors: | Wang Junjie Sun Xinchen Shen Wenjiang et al Cancer Therapy Center of Beijing Medical University Beijing |
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| Abstract: | Purpose: To study the apoptosis induced by Kang-Lai-Te injection (KLT)in renal cancer cell lines (GRC-1) Methods: The inhibitive effect of KLT on GRC-1 was measured by MTT Apoptosis was tested by TUNEL The expression of p53 and bcl-2 genes was determined by immunohistochemical staining Results: KLT could inhibit the GRC-1 cell growth, the IC50 value was 1931l/mlWhen it was exposed to 5l/ml and 10l/ml of KLT for 48 hours, the apoptotic percentages of renal cancer cell lines were 3130%and 8976%respectively, and it was less than 10%at the concentration of 1520l/ml The expression level of bcl-2 gene was lowered significantly at the concentration of 520l/ml than that of the control group Conclusion: KLT injection can induce apoptosis in renal cancer cells It may increase the p53 gene expression but inhibits the bcl-2 gene expression |
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| Keywords: | Kang-Lai-Te Apoptosis p53 bcl-2 |
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