胃良恶性疾病外周血淋巴细胞染色体脆性位点表达与胃癌的发生

目的：探讨胃良恶性疾病外周血淋巴细胞染色体脆性位点与胃癌发生的关系。方法：采用低叶酸培养条件，比较胃癌（１５例）与慢性浅表性胃炎和胃溃疡（各１５例）患者的外周血淋巴细胞染色体脆性位点表达率，差异有非常显著性意义。结果：胃癌组阳生个体表达例数有统计学意义的７个脆性位点５个与癌断裂点、癌基因居染色体同一区带，涉及到７个癌基因，表明与癌基因同位或相邻的脆性位点活跃表达在胃癌发生中起了极重要的作用。同一胃

Expression of Chromosome Fragile Site in Peripheral Lymphocytes in Benign and Malignant Gastric Diseases and Its Role in the Genesis of Gastric Cancer

The expression of chromosome fragile site (Fra) in peripheral lymphocytes (PBL) in patients with gastric cancer (GC) (15 cases), chronic gastritis (15 cases ) and gastric ulcer (15 cases) were examined by using low folate culture medium Significant differences were observed among these groups (P< 001) Five out of 7 statisticaly significant fragile sites were at the same band with the cancer breakpoints and oncogene in chromosome (in which 7 oncogenes were involved) That means active expression of fragile sites in the same site or neighbourhood of the oncogenes plays an important role in the genesis of gastric cancer The aberration of many oncogenes discovered in a same case of GC indicates that genesis and development of GC are the outcome of multi-gene injuries When aberration of 1q21 and other relevant loci in chromosome are seen in benign gastric diseases, the possibility of GC should be considered