| 端粒酶活性与白血病细胞系的分化 |
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| 引用本文： | 张莉萍,蒋纪恺,刘小珊. 端粒酶活性与白血病细胞系的分化[J]. 中国肿瘤临床, 1999, 22(3): 178-181 |
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| 作者姓名： | 张莉萍 蒋纪恺 刘小珊 |
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| 作者单位： | ：刘祥贵 张莉萍 重庆医科大学第二临床学院（重庆市400010）;刘小珊 重庆医科大学第一临床学院;蒋纪恺 张彦 许湘儒 刘北忠 何渝军 刘祥贵 康格非 重庆医科大学临床生化 |
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| 基金项目： | 国家自然科学基金!39670881 |
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| 摘 要： | 为了探讨白血病细胞的分化和端粒酶活性改变的关系。方法采用ＰＣＲ－ＬＩＳＡ方法检测白血病细胞株Ｋ－５６２和ＨＬ－６０诱导分化前后的端粒酶活性。结论分化是细胞自身调控的过程，端粒酶活性的高低与细胞所处的分化状态密切相关。
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| 关 键 词： | 端粒酶 活性 白血性 细胞系 PCRELISA |
Telomerase Activity and Differentiation of Leukemia Cells |
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| Zhang Liping,Jiang Jikai,Liu Xiaoshan,et al. Telomerase Activity and Differentiation of Leukemia Cells[J]. Chinese Journal of Clinical Oncology, 1999, 22(3): 178-181 |
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| Authors: | Zhang Liping Jiang Jikai Liu Xiaoshan et al |
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| Abstract: | Purpose: To study the relationship between differentiation of leukemia cells andalteration of telomerase activity. Methods: Telomerase activity was evaluated by PCR - ELISA duringand following induced differentiation in K - 562 and HL -60 cells. Results: Untreated K -562 andHL - 60 cells had higher telomerase activity which was significantly inhibited in K - 562 cells treated withMatrine and in HL - 60 cells treated with retinoic acid. The differentiation - inducing agents which hadnot directly inhibit telomerase activity indicated that the inhibition of telomerase activity responded to theinduction of differentiation and the loss of telomerase activity was closely associated with the state of thecellular differentiation. Conclusion: Cellular differentiation is a self - regulation process of the cells and,telomerase activity is associated with the state of cell differentiation. |
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| Keywords: | Telomerase activity PCR - ELISA Leukmia cell lines |
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