造血干细胞移植植入存活检定技术的应用性研究

目的 探讨荧光标记短串联重复(STR)复合扩增技术在造血干细胞移植(SCT)存活检定中的应用价值。方法 用荧光标记引物对15个STR位点和1个性别位点牙釉质蛋白基因(amelogenin)进行聚合酶链反应(PCR)复合扩增，再用DNA自动测序仪对PCR产物进行分离及分型，并对30例移植后病人做存活鉴定检测。结果 有20例移植后病人的15个STR位点的分型完全表现供者源性，与受者移植前不同；3例表现为供／受体干细胞混合嵌合状态，7例未表现出供者源性细胞在受者体内的植入。结论 荧光标记STR复合扩增体系特异性强，个体识别力高，检测灵敏、快速、简便，可作为SCT存活的可靠指标。

A study on the technology for detection of engraftment after stem cell transplantation

Objective To evaluate the fluorescent multiplex amplification of STRs using for detection of engraftment after stem cell transplantation(SCT).Methods 30 cases were analyzed by the fluorescent multiplex amplification of 15 STR loci and Amelogenin gene. PCR products were separated and typed using DNA Sequencer. Results In 20 cases, the evidences of survival of engraftment of originated cells from donors were obtained in recipients. In 3 cases, the evidences of mixed chimerism were observed and in 7 cases, the evidences of no survival of engraftment of originated cells from donors were shown. Conclusion The system of fluorescent multiplex amplification of STRs had a high power of discrimination and were highly recognizable. Its use in the detection of engraftment after SCT is reliable, sensitive and simple.