达原饮中芍药苷和黄芩苷在大鼠体内药动学研究

目的:建立同时测定大鼠血浆中芍药苷和黄芩苷浓度的高效液相色谱-串联质谱(LC-MS/MS)方法,并研究大鼠体内达原饮的药代动力学。方法:采用蛋白质沉淀法处理血浆样品,色谱柱为Inersil ODS柱(4.6 mm×50 mm,5μm),流动相为水-乙腈梯度洗脱,柱温为35℃;流速为0.5 mL·min^-1。质谱采用电喷雾离子源(ESI),选择离子反应监测(SRM)模式。结果:该方法准确度、精密度、回收率和基质效应均符合生物基质样品测试要求,芍药苷和黄芩苷的线性范围均为5~2000 ng·mL^-1。芍药苷和黄芩苷的药代动力学参数AUC为(2.300374×10^4±7.42703×10^3)、(2.3423881×10^5±6.678954×10^4)ng·mL^-1·min;T1/2z为(126.65±34.65)、(168.18±46.44)min;Tmax为(30.00±0)、(27.50±6.12)min;Cmax为(170.44±53.76)、(1645.42±464.35)ng·mL^-1。结论:建立的大鼠血浆中芍药苷和黄芩苷浓度测定的LC-MS/MS方法简便易行,准确灵敏,适用于达原饮在大鼠体内的药代动力学研究。

In vivo pharmacokinetic study of peoniflorin and baicalin from Dayuanyin in rats

Objective:To establish a liquid chromatograph-mass spectrometer/mass spectrometry(LC-MS/MS)method to simultaneously determine the plasma concentration of peoniflorin and baicalin from Dayuanyin in rats,and explore the in vivo pharmacokinetics of Dayuanyin in rats.Methods:The plasma sample was handled by protein precipitation.The column was Inersil ODS column(4.6 mm×50 mm,5μm).The mobile phase consisted of water and acetonitrile gradient eluting at 0.5 mL·min-1.The column temperature was set at 35℃.The electrospray ion source(ESI)and selective the ion reaction monitoring(SRM)mode were used.Results:The precision,accuracy,mean recoveries and the matrix effects met the requirements of the biological sample measurement.The linear ranges of peoniflorin and baicalin were both from 5 ng·mL^-1 to 2000 ng·mL^-1.The pharmacokinetic parameters of peoniflorin and baicalin were as follows:AUC:(2.300374×10^4±7.42703×10^3)ng·mL^-1·min and(2.3423881×10^5±6.678954×10^4)ng·mL^-1·min respectively;T1/2 z:(126.65±34.65)min and(168.18±46.44)min respectively;Tmax:(30.00±0)min and(27.50±6.12)min respectively;Cmax:(170.44±53.76)ng·mL^-1 and(1645.42±464.35)ng·mL^-1 respectively.Conclusion:The established LC-MS/MS method for determination of peoniflorin and baicalin in rats plasma was simple,accurate and sensitive,which was suitable for the pharmacokinetic study of peoniflorin and baicalin from Dayuanyin in rats in vivo.