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| 壳聚糖对大鼠Ⅲ度烧伤创面EGF和PDGF表达的影响 | |
| 引用本文： | 廖红,董志,傅洁民,朱毅,朱丽霞,刘全. 壳聚糖对大鼠Ⅲ度烧伤创面EGF和PDGF表达的影响[J]. 中国药理学通报, 2006, 22(11): 1375-1378 |
| 作者姓名： | 廖红 董志 傅洁民 朱毅 朱丽霞 刘全 |
| 作者单位： | 1. 重庆医科大学药理教研室,生物化学和分子药理学重点实验室,重庆,400016 2. 重庆医药工业研究院,重庆,400061 3. 海南省药品检验所,海南,海口,570216 |
| 摘 要： | 目的研究壳聚糖对大鼠Ⅲ度烧伤创面表皮生长因子(EGF)和血小板源性生长因子(PDGF)表达的影响。方法W istar大鼠分为1%壳聚糖组(W/V)、2%壳聚糖组(W/V)、4%壳聚糖组(W/V)、成纤维细胞生长因子(贝复剂)组(阳性对照组)、自然愈合组(模型组),制备成大鼠Ⅲ度烧伤模型,采用免疫组织化学SABC法、原位杂交法及图像分析法检测大鼠Ⅲ度烧伤创面EGF和EGFmRNA的表达;PDGF的活性用L929细胞生物法测定。结果4%壳聚糖组(W/V)对烧伤后7、14 d创面EGF的表达、对烧伤后3、7 d创面EG-FmRNA的表达有明显的提高;对烧伤后3、7、14 d创面PDGF的活性有明显的提高,与自然愈合组相比,差异均有显著性(P<0.05)。结论壳聚糖对大鼠Ⅲ度烧伤创面的EGF表达和PDGF活性有促进作用。 |
| 关 键 词： | 壳聚糖 表皮生长因子 血小板源性生长因子 烧伤 愈合 |
| 文章编号： | 1001-1978（2006）11-1375-04 |
| 收稿时间： | 2006-06-10 |
| 修稿时间： | 2006-06-102006-08-10 |
Effect of chitosan on EGF and PDGF in the wound of Ⅲ skin burns in rats |
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| LIAO Hong,DONG Zhi,FU Jie-ming,ZHU Yi,ZHU Li-xia,LIU Quan. Effect of chitosan on EGF and PDGF in the wound of Ⅲ skin burns in rats[J]. Chinese Pharmacological Bulletin, 2006, 22(11): 1375-1378 | |
| Authors: | LIAO Hong DONG Zhi FU Jie-ming ZHU Yi ZHU Li-xia LIU Quan |
| Affiliation: | 1. The Pharmacology Department of Chongqing University of Medical Science, Chongqing 400016, China; 2. Chongqing Phartmweutical Research Institute, Chongqing 400061, China 3. Hainan Provincial Institute for Drug Control,Haikou 570216 ,China |
| Abstract: | Aim To study the effect of chitosan on the expression of epidermal growth factor(EGF) and the activity of platelet-derived growth factor(PDGF) in the wound of Ⅲ skin burns in rats.Methods Wistar rats were randomly divided into 5 groups as follows: 1% chitosan(W/V) group,2% chitosan(W/V) group,4% chitosan(W/V) group,bFGF(basic fibroblast growth factor) group and the control group.Rats were made for Ⅲ skin burns.Immunohistochemisty,in situ hybridation and image analysis were employed to detect the expression of EGF and its mRNA in the wound of Ⅲ skin burns in rats;PDGF was determined by bioassay.Results Expression of EGF,EGF mRNA and PDGF in the wound of Ⅲ skin burns in rats using 4% chitosan(W/V) were significantly higher than those of other groups.Conclusion Chitosan could significantly promote expressions of EGF and PDGF during the wound healing of Ⅲ skin burns in rats. |
| Keywords: | chitosan epidermal growth factor platelet-derived growth factor burn wound healing |
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