前列腺癌和癌旁组织中雌激素受体α和β的表达及意义

目的 通过测定前列腺癌中癌组织、癌旁组织和良性前列腺增生组织中雌激素受体(estrogen receptor,ER)α和β的表达水平,探讨ER在前列腺腺癌发生、发展中的变化和作用机制.方法 采用超高敏链酶亲合素-过氧化物酶法检测28例前列腺腺癌标本中癌和癌旁组织以及29例良性前列腺增生组织中ERα和ERβ的表达水平,对比其在各组之间表达的差异,分析ERα和ERβ的表达水平与前列腺癌患者的Gleason评分、临床分期、年龄和TPSA的关系.结果 前列腺癌组织中ERα主要在间质细胞表达.ERα在前列腺癌组织、癌旁组织和良性前列腺增生组织上皮细胞的表达阳性率分别为0%、14%、24%,间质细胞阳性率分别为57%、68%、31%,组间比较差异有统计学意义(P＜0.05).前列腺癌上皮细胞和间质细胞均有ERβ的表达且差异无统计学意义(P＞0.05).ERβ在前列腺癌组织、癌旁组织和良性前列腺增生组织上皮细胞的表达阳性率分别为39%、64%、29%;间质细胞阳性率分别为50%、75%、79%,组间比较差异有统计学意义(P＜0.05).ERβ在不同Gleason评分前列腺癌组织中表达差异有统计学意义(P＜0.05).结论 前列腺癌中ERα主要在间质细胞表达;ERβ在上皮细胞和间质细胞均有表达.ERα和ERβ的表达水平在前列腺癌组织、癌旁组织和良性前列腺增生组织均存在差异.ER口与前列腺癌变发生和恶性程度相关.

Abstract：

Objective To investigate the expression of estrogen receptor (ER) α and β in human prostate cancer (PC), peri-cancer tissue and benign prostatic hyperplasia (BPH) tissue, and to discuss the role of estrogen receptor in prostate cancer. Methods The expression of ERα and ERβ in PC (n=28), peri-cancer tissue (n=28) and BPH (n=29) were detected by immunohistochemistry with En vision method. The ERα and ERβ expression were compared among different tissues by chisquare. The relationship between ER expression and related clinicopathologic features was statistically analyzed by spearman rank collection. Results ERα was localized dominantly in the stromal cell of PC. There were significant differences of the expression of ERα in PC, peri-cancer tissue and BPH tissue (epithelial cell 0%, 14%, 24%, P＜0. 05; stromal cell 57%, 68%, 31%,P＜0. 05). ERβ was localized in both epithelial and stromal cell of PC. There were significant differences of the expression of ERβ in PC, peri-cancer tissue and BPH tissue (epithelial cell 39%, 64%, 29%, P＜0.01; stromal cell 50%, 75%, 79%, P＜0.05). There was a significant difference of the expression of ERβ in different Gleason scores of PC tissue. Conclusions ERα is localized in the stromal cell of PC tissue.ERβ is localized in both epithelial and stromal cell of PC tissue. The ERβ might be related to the tumor differentiation of PC.