麝香保心丸对心力衰竭大鼠Th1和Th2型细胞因子水平的影响

目的 探讨麝香保心丸对心肌梗死后心力衰竭大鼠血清Th1型细胞因子、肿瘤坏死因子-α(TNF-α)、干扰素-γ(IFN-γ)和Th2型细胞因子白介素-4(IL-4)、白介索-10(IL-10)水平的影响.方法 结扎大鼠左冠状动脉前降支制作心肌梗死后心力衰竭模型,54只Wistar大鼠随机分为正常对照组、假手术组、心力衰竭模型对照组(模型组)、麝香保心丸小剂量组(小剂量组)、麝香保心丸大剂量组(大剂量组)和阳性药物对照组(阳性对照组),采用灌胃法给药.超声心动图检测用药前后大鼠心功能,放射免疫法测定血清TNF-α、IL-10水平,酶联免疫吸附法测定血清IFN-γ、IL-4水平.结果 用药前,假手术组与正常对照组左心室射血分数(LVEF)比较,差异无统计学意义;与正常对照组和假手术组比较,模型组、小剂量组大剂量组和阳性对照组大鼠LVEF降低(均P＜0.01);用药后,与假手术组比较,模型组的TNF-α和IFN-γ水平升高(P＜0.01);IL-4和IL-10水平降低(P＜0.01);与模型组比较,大剂量组的LVEF改善(P＜0.05),TNF-α和IFN-γ水平降低(P＜0.05,P＜0.01),IL-4和IL-10水平升高(P＜0.05),小剂量组的IL-10水平升高(P＜0.05),IFN-γ水平下降(P＜0.01);阳性对照组IL-10水平升高(P＜0.05),TNF-α和IFN-γ水平下降(P＜0.05,P＜0.01);小剂量组和阳性对照组的IL-4水平有升高趋势,LVEF有下降趋势,但差异均无统计学意义.结论 麝香保心丸治疗改善了心肌梗死后心力衰竭大鼠的左心功能,大鼠血清IL-4、IL-10水平升高,TNF-α、IFN-γ降低.

Abstract：

Objective To investigate the effects of Shexiangbaoxin pills (SXBXP) on the serum levels of T helper type 1 (Th1) cytokines including tumor necrosis factor-a (TNF-α),interferon-γ(IFN-γ) and T helper type 2 (Th2) cytokines incloding interleukin-4 (IL-4),interleukin-10 (IL-10)in rats with chronic heart failure (CHF).Methods The CHF models were established by left anterior descending coronary artery ligation.Fifty-four Wistar rats were randomly divided into six groups:control group,sham operation group,CHF model group,small dose SXBXP group,large dose SXBXP group and positive medicine group.Heart function was examined by echocardiography before and after therapy,the TNF-α and IL-10 levels were measured by radioimmunoassay,IFN-γ and IL-4 levels were measured by enzyme linked immunosorbent assay (ELISA).Results (1) Before therapy,there was no significant difference between control group and sham operation group in ejection fraction of left ventricle (LVEF).Compared with control group and sham operation group,the levels of LVEF were significantly decreased in CHF model group,small dose SXBXP group,large dose SXBXP group and positive medicine group (all P＜0.01).(2) After therapy,compared with sham operation group,the serum levels of TNF-α and IFN-γ were markedly increased,and IL-4 and IL-10 were significantly decreased in CHF model group (all P＜0.01 ).Compared with CHF model group,LVEF was significantly improved,serum levels of TNF-α and IFN-γ were markedly decreased,IL-4 and IL-10 were markedly increased in large dose SXBXP group (all P＜0.05).In small dose SXBXP group,serum levels of IFN-γ were markedly decreased and IL-10 were markedly increased (all P＜0.05).In positive medicine group,serum levels of TNF-α and IFN-γ were markedly decreased and IL-10 were markedly increased (all P＜0.05).In small dose SXBXP group and positive medicine group,serum levels of IL-4 increased and LVEF decreased,even though there was no significant difference compared with CHF model group.Conclusions The SXBXP therapy of rats with CHF induced by myocardial infarction improves the heart function,decreases the serum levels of TNF-α and IFN-γ,and increases serum levels of IL-4 and IL-10.

Effects of Shexiangbaoxin pills therapy on serum levels of T helper type 1 and 2 cytokines in rats with heart failure

Objective To investigate the effects of Shexiangbaoxin pills (SXBXP) on the serum levels of T helper type 1 (Th1) cytokines including tumor necrosis factor-a (TNF-α),interferon-γ(IFN-γ) and T helper type 2 (Th2) cytokines incloding interleukin-4 (IL-4),interleukin-10 (IL-10)in rats with chronic heart failure (CHF).Methods The CHF models were established by left anterior descending coronary artery ligation.Fifty-four Wistar rats were randomly divided into six groups:control group,sham operation group,CHF model group,small dose SXBXP group,large dose SXBXP group and positive medicine group.Heart function was examined by echocardiography before and after therapy,the TNF-α and IL-10 levels were measured by radioimmunoassay,IFN-γ and IL-4 levels were measured by enzyme linked immunosorbent assay (ELISA).Results (1) Before therapy,there was no significant difference between control group and sham operation group in ejection fraction of left ventricle (LVEF).Compared with control group and sham operation group,the levels of LVEF were significantly decreased in CHF model group,small dose SXBXP group,large dose SXBXP group and positive medicine group (all P＜0.01).(2) After therapy,compared with sham operation group,the serum levels of TNF-α and IFN-γ were markedly increased,and IL-4 and IL-10 were significantly decreased in CHF model group (all P＜0.01 ).Compared with CHF model group,LVEF was significantly improved,serum levels of TNF-α and IFN-γ were markedly decreased,IL-4 and IL-10 were markedly increased in large dose SXBXP group (all P＜0.05).In small dose SXBXP group,serum levels of IFN-γ were markedly decreased and IL-10 were markedly increased (all P＜0.05).In positive medicine group,serum levels of TNF-α and IFN-γ were markedly decreased and IL-10 were markedly increased (all P＜0.05).In small dose SXBXP group and positive medicine group,serum levels of IL-4 increased and LVEF decreased,even though there was no significant difference compared with CHF model group.Conclusions The SXBXP therapy of rats with CHF induced by myocardial infarction improves the heart function,decreases the serum levels of TNF-α and IFN-γ,and increases serum levels of IL-4 and IL-10.