急性髓系白血病SFRP4基因启动子区异常甲基化研究

目的研究急性髓系白血病中SFRP4基因启动子区的异常甲基化状态,探讨其与急性髓系白血病发生的关系。方法应用甲基化特异性PCR法对99例急性髓系白血病患者的骨髓或外周血进行SFRP4基因启动子区甲基化状况检测,并以70例门诊普通患者的外周血作为对照。结果 99例急性髓系白血病患者中有17例SFRP4基因的启动子区发生了甲基化,甲基化率为17.2%;而正常对照组中未检出SFRP4基因启动子区的甲基化。SFRP4基因在急性髓系白血病患者中的甲基化率极显著的高于正常对照(P<0.001)。SFRP4基因的甲基化状态与急性髓系白血病患者的年龄、性别及临床分型间均未出现显著相关性(P>0.05)。结论 SFRP4基因启动子区的异常甲基化与急性髓系白血病的发生有相关性,SFRP4基因的甲基化可能是引起急性髓系白血病的分子机制之一。

Study of abnormal methylation in the promoter region of SFRP4 gene in acute myeloid leukemia

Objective To investigate the abnormal methylation status in the promoter region of SFRP4 gene in acute myeloid leukemia （AML）, and explore whether the abnormal methylation of SFRP4 gene is associated with acute myeloid leukemia. Methods MSP method was applied to examine the promoter methylation of SFRP4 gene in 99 bone marrow or peripheral blood samples of AML patients. As controls, 70 normal peripheral blood samples from volunteers of general outpatients were examined. Results In 99 samples of AML patients, 17 samples （17. 2% ） showed SFRP4 gene methylation, while no one showed SFRP4 gene methylation in 70 controls. There was an extremely significant difference of the SFRP4 gene methylation frequency between AML patients and controls （P 〈 0. 001 ）. The methylation status of SFRP4 gene was not associated with age, gender and the clinical classification of AML patients （ P 〉 0. 05 ）. Conclusions The abnormal methylation in the promoter region of SFRP4 gene was correlated with AML. The methylation of SFRP4 gene may be one of the molecular mechanisms of AML.