正己烷对人体血清髓鞘P0与P2蛋白抗体的影响

目的 了解正己烷对人体血清髓鞘P0蛋白抗体与P2蛋白抗体表达的影响.方法 选取接触正己烷1年以上的工人269名作为接触组,同时选取未接触正己烷的工人104名作为对照组,测定工人尿中2,5-己二酮的含量,并依据WS/T243-2004<职业接触正己烷的生物限值>将含量超出和未超出生物限值的工人分别设为高接触组和低接触组,进一步使用酶联免疫吸附法测定工人血清中P0抗体、P2抗体的水平.结果 正己烷接触组工人尿中2,5-己二酮含量均值为(3.10±1.35)mg/L,对照组尿中2,5-己二酮含量均低于最低检出限.接触组工人血清中P0抗体(IgG、IgM)和P2抗体(IgG、IgM)表达均明显增加,与对照组相比,差异有统计学意义(P＜0.01),但接触组之间差异无统计学意义(P＞0.05).结论 正己烷可导致人体血清中P0抗体以及P2抗体升高,血清中P0抗体以及P2抗体可作为正己烷接触的易感性标志物,这将有助于正己烷职业中毒的早期发现与治疗,同时也为制定卫生标准提供新的理论依据.

Abstract：

Objective Exploring the effects of n-hexane on expression of serum myelin proteins in occupational exposure workers, and finding the early biomarker of n-hexane exposure. Methods In the study, 373 subjects were recruited, 269 exposure workers (work experience of more than 1 year)and 104 nonexposure workers were selected. Firstly examined the level of urinary 2,5-hexanedione in the two groups,based on urinary 2,5-hexanedione biological limit value (4 mg/L), the exposed group was divided into highexposed group and low-exposed group. And then collected blood samples and extracted serum. Human peripheral myelin protein zero (PO) antibody (IgG, IgM) and human peripheral myelin protein two(P2)antibody (IgG, IgM) analysis was performed according to ELISA kit. Results The concentration of urinary 2,5-hexanedione in the exposed group was (3.10± 1.35 ) mg/L. The level of PO antibody (IgG, IgM ) and P2 antibody (IgG, IgM) in the high-exposed group and low-exposed group were both higher than that in the controls (P＜0.01). Conclusion P0 antibody and P2 antibody could be used as the early biomarkers of nhexane exposure, which not only evaluate the occupational hazards in the early, but also provide the policy maker with scientific evidence.

Effects on serum myelin proteins of n-hexane exposure

Objective Exploring the effects of n-hexane on expression of serum myelin proteins in occupational exposure workers, and finding the early biomarker of n-hexane exposure. Methods In the study, 373 subjects were recruited, 269 exposure workers (work experience of more than 1 year)and 104 nonexposure workers were selected. Firstly examined the level of urinary 2,5-hexanedione in the two groups,based on urinary 2,5-hexanedione biological limit value (4 mg/L), the exposed group was divided into highexposed group and low-exposed group. And then collected blood samples and extracted serum. Human peripheral myelin protein zero (PO) antibody (IgG, IgM) and human peripheral myelin protein two(P2)antibody (IgG, IgM) analysis was performed according to ELISA kit. Results The concentration of urinary 2,5-hexanedione in the exposed group was (3.10± 1.35 ) mg/L. The level of PO antibody (IgG, IgM ) and P2 antibody (IgG, IgM) in the high-exposed group and low-exposed group were both higher than that in the controls (P＜0.01). Conclusion P0 antibody and P2 antibody could be used as the early biomarkers of nhexane exposure, which not only evaluate the occupational hazards in the early, but also provide the policy maker with scientific evidence.