大蒜油及大鼠周龄和性别对正己烷体内代谢的影响

目的 探讨大蒜油及周龄、性别对正己烷在大鼠体内代谢的影响.方法 以Wistar大鼠为实验动物,(1)灌胃染毒:正己烷组(3000 mg/kg正己烷),大蒜油干预组(染毒前1 h予80 mg/kg大蒜油灌胃),8、12、16、20、24、28、32 h断尾取血.(2)腹腔注射染毒:正己烷组(1000 mg/kg正己烷),大蒜油干预组(染毒药前1 h予80 mg/kg大蒜油灌胃),8、12、16、20、24、28 h断尾取血.(3)6、8、10周龄(均为7只)雄性大鼠予正己烷3000 mg/kg灌胃,染毒后16、20、24 h断尾取血.(4)8周龄大鼠雌、雄各7只,予正己烷3000 mg/kg灌胃,染毒后16、20、24、28 h断尾取血.气相色谱法测定血清中2,5-己二酮浓度,比较给和不给大蒜油、不同周龄、不同性别大鼠血清中2,5-己二酮浓度.结果 (1)灌胃染毒:正己烷组、大蒜油干预组血清中2,5-己二酮浓度分别在20和24 h达到峰值,分别为19.2和12.3μg/ml,在峰值及之前时间点大蒜油干预组血清中2,5-己二酮浓度明显降低,但峰值后的消除过程明显减缓.(2)腹腔注射染毒:大蒜油对血清中2,5-己二酮浓度的影响与正己烷灌胃途径基本相同,两组分别在12和16 h达到峰值,分别为15.0和6.7 μg/ml.(3)周龄:16 h时6、8、10周龄大鼠血清中2,5-己二酮浓度分别为25.5、15.0、12.8μg/ml,8、10周龄与6周龄的差异有统计学意义(P＜0.05或P＜0.01);20 h时6、8、10周龄大鼠血清中2,5-己二酮浓度分别为24.7、18.3、15.0 μg/ml,10周龄与6周龄的差异有统计学意义(P＜0.05);24 h时6、8、10周龄大鼠血清中2,5-己二酮浓度分别为11.0、14.7、8.1 μg/ml,10周龄与8周龄的差异有统计学意义(P＜0.05).(4)性别:16 h时雄性、雌性大鼠血清中2,5-己二酮浓度分别为22.5、17.2 μg/ml,差异有统计学意义(P＜0.05);在20、24、28 h雄性、雌性大鼠血清中2,5-己二酮浓度分别为27.6、22.9μg/ml,24.6、19.1 μg/ml,19.1、13.8μg/ml,不同性别间的差异均无统计学意义(P＞0.05).结论 大蒜油可明显减少正己烷在大鼠体内代谢物2,5-己二酮的生成量;低周龄的动物正己烷的代谢能力高于高周龄的动物.

Abstract：

Objective To investigate effects of garlic oil (GO),age and sex on n-hexane metabolism in rats. Methods The Wistar rats were used as experimental animals. (1) Intragastric administration: nhexane group (3000 mg/kg n-hexane),GO treated group (80 mg/kg GO ig. an hour earlier than 3000 mg/kg n-hexane), then blood was taken from tails of rats at 8, 12, 16, 20, 24, 28, 32 h points after n-hexane administration. (2)Intraperitoneal injection: n-hexane group ( 1000 mg/kg n-hexane), GO treated group (80 mg/kg GO ig. an hour earlier than 1000 mg/kg n-hexane), then took blood was taken from tails of rats at 8, 12,16, 20, 24, 28 h points after n-hexane injection. (3) 7 rats each group of 6, 8, 10 weeks age were administrated by 3000 mg/kg n-hexane intragastrically, then were taken blood from tails at 16, 20, 24 h points after administration.(4) 7 male and 7 female rats of 8 weeks age were administrated by 3000 mg/kg n-hexane intragastrically, then were taken blood from tails at 16, 20, 24, 28 h points after administration. The gas chromatography was used to determine the metabolite 2, 5-hexanedione concentration of n-hexane in serum and 2, 5-hexanedione concentration was compared between GO and no GO treated rats, different ages and different sexes. Results ( 1 )Intragastric administration: 2, 5-hexanedione concentrations in serum of n-hexane group and GO treated group had the peak 19.2 and 12.3 μg/ml at 20 h and 24 h points. Compared with nhexane group, the serum 2, 5-hexanedione concentration of GO treated group was lower at time points prior to peak and 2, 5-hexanedione eliminating process was slower after peak. (2) Intraperitoneal injection: effects of GO on the serum 2, 5-hexanedione concentrations was very similar to intragastric administration, 2, 5-hexanedione concentrations in serum of n-hexane group and GO treated group had the peak 15.0 and 6.7μg/ml at 12 h and 16 h points.(3) Comparison of the serum 2, 5-hexanedione concentrations of different weeks age rats: The serum 2, 5-hexanedione concentrations of 6, 8, 10 weeks age rats were 25.5, 15.0, 12.8μg/ml each (8, 10 weeks age significantly lower than 6 weeks age) at 16 h point; at 20 h point, they were 24.7, 18.3, 15.0 μg/ml each ( 10 weeks age significantly lower than 6 weeks age); at 24 h point, they were 11.0, 14.7, 8.1 μg/ml each (10 weeks age significantly lower than 8 weeks age). (4) Comparisons of the serum 2, 5-hexanedione concentrations of different sex rats: the serum 2, 5-hexanedione concentrations of male and female rats were 22.5, 17.2 μg/ml each at 16 h point (different significantly); at 20, 24, 28 hpoints, they were 27.6, 22.9 μg/ml, 24.6,19.1 μg/ml, 19.1, 13.8 μg/ml each (different non-significantly).Conclusion GO reduces production of 2, 5-hexanedione in serum generated by n-hexane in rats; the metabolic capacity of low age rats on n-hexane is stronger than high age ones.

Effects of garlic oil, age and sex on n-hexane metabolism in rats

Objective To investigate effects of garlic oil (GO),age and sex on n-hexane metabolism in rats. Methods The Wistar rats were used as experimental animals. (1) Intragastric administration: nhexane group (3000 mg/kg n-hexane),GO treated group (80 mg/kg GO ig. an hour earlier than 3000 mg/kg n-hexane), then blood was taken from tails of rats at 8, 12, 16, 20, 24, 28, 32 h points after n-hexane administration. (2)Intraperitoneal injection: n-hexane group ( 1000 mg/kg n-hexane), GO treated group (80 mg/kg GO ig. an hour earlier than 1000 mg/kg n-hexane), then took blood was taken from tails of rats at 8, 12,16, 20, 24, 28 h points after n-hexane injection. (3) 7 rats each group of 6, 8, 10 weeks age were administrated by 3000 mg/kg n-hexane intragastrically, then were taken blood from tails at 16, 20, 24 h points after administration.(4) 7 male and 7 female rats of 8 weeks age were administrated by 3000 mg/kg n-hexane intragastrically, then were taken blood from tails at 16, 20, 24, 28 h points after administration. The gas chromatography was used to determine the metabolite 2, 5-hexanedione concentration of n-hexane in serum and 2, 5-hexanedione concentration was compared between GO and no GO treated rats, different ages and different sexes. Results ( 1 )Intragastric administration: 2, 5-hexanedione concentrations in serum of n-hexane group and GO treated group had the peak 19.2 and 12.3 μg/ml at 20 h and 24 h points. Compared with nhexane group, the serum 2, 5-hexanedione concentration of GO treated group was lower at time points prior to peak and 2, 5-hexanedione eliminating process was slower after peak. (2) Intraperitoneal injection: effects of GO on the serum 2, 5-hexanedione concentrations was very similar to intragastric administration, 2, 5-hexanedione concentrations in serum of n-hexane group and GO treated group had the peak 15.0 and 6.7μg/ml at 12 h and 16 h points.(3) Comparison of the serum 2, 5-hexanedione concentrations of different weeks age rats: The serum 2, 5-hexanedione concentrations of 6, 8, 10 weeks age rats were 25.5, 15.0, 12.8μg/ml each (8, 10 weeks age significantly lower than 6 weeks age) at 16 h point; at 20 h point, they were 24.7, 18.3, 15.0 μg/ml each ( 10 weeks age significantly lower than 6 weeks age); at 24 h point, they were 11.0, 14.7, 8.1 μg/ml each (10 weeks age significantly lower than 8 weeks age). (4) Comparisons of the serum 2, 5-hexanedione concentrations of different sex rats: the serum 2, 5-hexanedione concentrations of male and female rats were 22.5, 17.2 μg/ml each at 16 h point (different significantly); at 20, 24, 28 hpoints, they were 27.6, 22.9 μg/ml, 24.6,19.1 μg/ml, 19.1, 13.8 μg/ml each (different non-significantly).Conclusion GO reduces production of 2, 5-hexanedione in serum generated by n-hexane in rats; the metabolic capacity of low age rats on n-hexane is stronger than high age ones.