| 首乌藤多糖提取方法比较 |
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| 引用本文： | 于雷,王保奇,董晶晶,杨云. 首乌藤多糖提取方法比较[J]. 中国实验方剂学杂志, 2012, 18(7): 38-40 |
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| 作者姓名： | 于雷 王保奇 董晶晶 杨云 |
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| 作者单位： | 河南中医学院药学院,郑州,450008 |
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| 基金项目： | 郑州市金水区科技攻关项目(20093534) |
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| 摘 要： | 目的:比较不同提取方法对首乌藤多糖提取效率的影响.方法:采用苯酚-硫酸法测定多糖含量,以粗多糖得率和多糖质量分数为评价指标,对首乌藤多糖不同提取方法进行研究.结果:6种方法中以氢氧化钠提取方法的效果最优,粗多糖得率和多糖质量分数可达25.13％和21.46％.结论:氢氧化钠提取首乌藤多糖是一种效率高且稳定可行的提取方法.
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| 关 键 词： | 首乌藤多糖 水提 碱提 酶提 酶-水提 |
| 收稿时间： | 2011-11-18 |
Comparison of Extraction Method of Caulis Polygoni Multiflori Polysaccharide |
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| YU Lei,WANG Bao-qi,DONG Jing-jing and YANG Yun. Comparison of Extraction Method of Caulis Polygoni Multiflori Polysaccharide[J]. China Journal of Experimental Traditional Medical Formulae, 2012, 18(7): 38-40 |
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| Authors: | YU Lei WANG Bao-qi DONG Jing-jing YANG Yun |
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| Affiliation: | College of Pharmacy, Henan University of Traditional Chinaese Medicine, Zhengzhou 450008, China;College of Pharmacy, Henan University of Traditional Chinaese Medicine, Zhengzhou 450008, China;College of Pharmacy, Henan University of Traditional Chinaese Medicine, Zhengzhou 450008, China;College of Pharmacy, Henan University of Traditional Chinaese Medicine, Zhengzhou 450008, China |
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| Abstract: | Objective: To compare effect of different extraction methods to extraction efficiency of polysaccharide from Caulis Polygoni Multiflori. Method: The content of polysaccharide was determined by phenol-sulfuric acid method,study on different extraction methods for polysaccharide from Caulis Polygoni Multiflori with the content and yield of polysaccharide as indexes. Result: Sodiumhydroxide extraction method was the best by comparing with other five methods,yield and the content of polysaccharide were up to 25.13%,21.46%,respectively. Conclusion: Sodiumhydroxide extraction was proved to be a high efficiency and stable method for extraction of polysaccharide from Caulis Polygoni Multiflori. |
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| Keywords: | polysaccharide from Caulis Polygoni Multiflori water extraction alkali extraction enzyme extraction enzyme-water extraction |
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