Chimerism monitoring by VNTRs polymorphism analysis in a patient who received allogenic bone marrow transplantation] |
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| Authors: | S Banno M Nitta A Wakita O Iwaki G Takeuchi K Takada Y Mitomo M Yamamoto |
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| Affiliation: | Second Department of Internal Medicine, Nagoya City University Medical School. |
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| Abstract: | A 39-year-old female diagnosed as acute myelogenous leukemia received allogenic bone marrow transplantation (BMT) pre-conditioned with busulfan and cyclophosphamide regimen from her HLA identical sibling. To distinguish donor and recipient cells, we analyzed variable numbers of tandem repeats (VNTRs) polymorphisms using a YNH-24 probe by Southern blot hybridization. VNTRs polymorphism analysis documented the engraftment of donor cells, relapse of recipient cells, and mixed hematopoietic chimerism. Assessment of the chimerism state is important for determining the prognosis of patients undergoing BMT, and VNTRs polymorphisms analysis is very useful for identifying the chimerism state. |
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