氨茶碱透皮制剂的穴位效应研究

目的研究氨茶碱透皮制剂穴位给药后的穴位效应,为穴位给药提供实验依据.方法采用透射电镜进行皮肤结构的形态学观察;I125放射免疫法测定血浆环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMP)的含量;双抗体夹心法(ELISA法)测定血清白细胞介素5(IL-5)的含量.结果与非穴位皮肤结构相比,穴位皮肤角质层细胞脱屑增加,脂质双分子层致密性改变,产生褶皱;各组给药后,血浆cAMP升高, cGMP降低,二者比值下降.其中,肺俞穴与膻中穴给药组可在2 h内使cAMP值趋于正常,在7 h内使cGMP和cAMP/cGMP比值趋于正常,小于非穴位给药组需要的时间(均为8 h以上).与哮喘对照组相比,肺俞组、非穴位组均可降低血清IL-5水平(P<0.05), 前者的影响大于后者,两者相比有显著性差异(P<0.05).结论氨茶碱透皮制剂穴位给药可产生显著的免疫调整作用.

Investigation into the Effective Responses of the Acupoints to Transdermal Aminophylline Preparation

Objective To investigate the effective responses of the acupoints to transdermal aminophylline preparation in the rabbits in order to provide experimental data for the acupoint administration. Method TEM was used to carry out the morphological observation of skin structures. I 125 radioimmunoassay was used to detect the levels of plasma cAMP and cGMP. ELISA was used to detect the level of serum IL-5.Results Compared with the skin structures at non-acupoints,an increase in cell desquamation of the stratum corneum,and changes in the density of the lipid bimolecular layer along with the formation of folds,were found in the skin structures at acupoints. After administration,the level of cAMP increased,and the level of cGMP decreased,and the ratio of cAMP/cGMP decreased as well in each experimental group. However,the level of cAMP tended to be normal in 2 hours and the ratio of cAMP/cGMP tended to be normal in 7 hours in the Feishu group and Tanzhong group while the same results showed in the non-acupoint group in over 8 hours,respectively. As compared with that in the asthma control group,the level of serum IL-5 was lowered in both the Feishu group and non-acupoint group ( P <0.05),and the effect was stronger in the Feishu group than in the non-acupoint group ( P <0.05). Conclusion The acupoint administration of transdermal aminophylline preparation can produce obvious immunoregulatory effect.