肺癌血管内皮生长因子表达及其与血管新生和肿瘤细胞增殖关系的研究

目的　探讨肺癌血管内皮生长因子（VEGF）的表达及其与血管新生、肿瘤细胞增殖的关系。方法　63例肺癌患者手术切除标本，应用免疫组织化学技术，抗VEGF165单克隆抗体（单抗）检测VEGF表达，抗CD34单抗测定肿瘤内微血管密度（iMVD）以反映血管新生，抗增殖细胞核抗原（PCNA）单抗测定肿瘤细胞增殖指数。结果　32例（50.8%）VEGF呈阳性表达，iMVD为4～138.7（M=36）/×400，PCNA为0～92%（M=25.03%）；VEGF评分为高级的肺癌组织iMVD高于VEGF为阴性和低级的肺癌组织（P＜0.01）,iMVD与VEGF表达呈正相关（r

Association of vascular endothelial growth factor expression with angiogenesis and tumor cell proliferation in human lung cancer

OBJECTIVE: To evaluate the expression of vascular endothelial growth factor (VEGF) and the relation with angiogenesis and tumor cell proliferation in human lung cancer. METHODS: Expression of VEGF, angiogenesis, tumor cell proliferation in 63 cases of human lung cancer were examined immunohistochemically. Anti-VEGF(165)-Ab3 was used for VEGF expression, a monoclonal antibody directed against CD(34) to identify intratumoral microvessel density (iMVD) and the proliferating cell nuclear antigen (PCNA) as a marker of proliferating tumor cells. RESULTS: Positive staining for VEGF was obtained in 32 of the 63 cases (50.8%), iMVD varied from 4 to 138.7 (M = 36)/x 400 and the proportion of PCNA positive cells varied from 0 to 92% (M = 25.03%). iMVD in high-grade VEGF tumors was significantly higher than that in VEGF-negative tumors and low-grade VEGF tumors. PCNA labeling proliferation index in small cell lung cancer was higher than that in non-small cell lung cancer. iMVD significantly increased with increasing VEGF expression. CONCLUSION: There is VEGF expression in lung cancer. VEGF expression is significantly associated with iMVD, but there is no association between iMVD or VEGF expression and PCNA expression. There is no association between VEGF expression and histological classification and clinical stages.