阿托伐他汀对高血压大鼠动脉血压和血管平滑肌细胞离子泵活性的影响

目的探讨阿托伐他汀对自发性高血压大鼠的动脉血压及血管平滑肌细胞离子泵活性的影响。方法选用12周龄自发性高血压大鼠12只,随机分为阿托伐他汀治疗组(简称阿托伐他汀组,n=6)和蒸馏水组(n=6),并以正常血压大鼠作为对照组。阿托伐他汀组大鼠给以阿托伐他汀50mg(kg·d)]加适量蒸馏水灌胃12周。观察给药前后大鼠尾动脉血压的变化,测定大鼠血清总胆固醇、甘油三酯和低密度脂蛋白胆固醇浓度,以及胸主动脉平滑肌细胞Na K ATP酶和Ca2 Mg2 ATP酶活性。结果阿托伐他汀组动脉血压显著低于蒸馏水组(161.8±9.9比192.9±10.4,P<0.05);阿托伐他汀组大鼠胸主动脉平滑肌细胞Na K ATP酶和Ca2 Mg2 ATP酶活性明显高于蒸馏水组(5.20±0.54比3.06±0.42,P<0.01;4.62±0.35比2.98±0.17,P<0.05),略低于对照组,而蒸馏水组则显著低于对照组(3.06±0.42比5.92±0.31,P<0.01;2.98±0.17比4.86±0.26,P<0.01)。Na K ATP酶活性、Ca2 Mg2 ATP酶活性与血压呈显著负相关(r=-0.426、r=-0.359,P<0.01)。结论长期应用阿托伐他汀可以显著降低自发性高血压大鼠血压。阿托伐他汀可能通过增高血管平滑肌细胞离子泵活性而影响血压形成的过程。

Effects of Atorvastatin on Aortic Blood Pressure and Ion Pump Activities of Vascular Smooth Muscle Cell in Spontaneously Hypertensive Rats

Aim To evaluate the effects of atorvastatin on aortic blood pressure and ion pump activities of vascular smooth muscle cell in spontaneously hypertensive rats (SHR). Methods Twelve twelve -week-old SHR were randomized into atorvastatin treated group (ATV group, n=6)and distilled water group (DW group,n=6),and Wistar-Kyoto rats (WKY)as normal controls. Atorvastatin and appropriate amounts of distilled water were administered to ATV group for 12 weeks by gavage. Blood pressure of caudal artery was examined before and after treatment,serum titres of TC, TG, LDLC, activities of Na+-K+-ATPase and Ca 2+-Mg 2+-ATPase of thoracic aorta smooth muscle cell were measured. Results Blood pressure in ATV group was much lower than that in DW group(161.8±9.9 vs 192.9±10.4,p<0.05). Activities of Na+-K+-ATPase and Ca 2+-Mg 2+-ATPase of thoracic aorta smooth muscle cell were remarkedly higher in both ATV group and WKY group than those in DW group (5.20±0.54 vs 3.06±0.42,p<0.01;4.62±0.35 vs 2.98±0.17,p<0.05),(5.92±0.31 vs 3.06±0.42, p<0.01; 4.86±0.26 vs 2.98±0.17,p<0.01). Activities of Na+-K+-ATPase and Ca 2+-Mg 2+-ATPase of thoracic aorta smooth muscle cell were negatively relative to blood pressure(r=-0.426,r=-0.359,p<0.01). Conclusion Long-term administration of atorvastatin can effectively reduce blood pressure by elevating the ion pump activities of vascular smooth muscle cell.