| Replication-incompetent Adenovirus Vector-mediated MDA-7/IL-24 Selectively Induces Growth Suppression and Apoptosis of Hepatoma Cell Line SMMC-7721 |
| |
| 作者姓名： | 王从俊 薛新波 易继林 吴在德 陈堃 郑建伟 吉文伟 于愿 |
| |
| 作者单位： | Department of Biliary and Pancreatic Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Biliary and Pancreatic Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Biliary and Pancreatic Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Biliary and Pancreatic Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Biliary and Pancreatic Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Biliary and Pancreatic Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Biliary and Pancreatic Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Biliary and Pancreatic Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology，Wuhan 430030 China，Wuhan 430030 China，Wuhan 430030 China，Wuhan 430030 China，Wuhan 430030 China，Wuhan 430030 China，Wuhan 430030 China，Wuhan 430030 China |
| |
| 基金项目： | Acknowledgements The authors are grateful to Prof. Paul. B. Fisher, Columbia University, USA, for his instruction, and to Prof. Jian-hua Zhou, Laboratory of Nephropathy, Department of Pediatrics, Tongji Hospital, Tongji Medical College, HUST, China, for his technical instruction and assistance. |
| |
| 摘 要： | In order to investigate the effect of replication-incompetent adenovirus vector expressing MDA-7/IL-24 on tumor growth and apoptosis of human hepatocellular carcinoma （HCC） cell line SMMC-7721 and normal liver cell line L02, the recombinant replication-incompetent Ad.mda-7 virus vector was constructed and infected into the HCC cell line SMMC-7721 and normal liver cell line L02. RT-PCR was performed to examine the expression of MDA-7 mRNA. The concentrations of MDA-7/IL-4 in culture superuatants were determined by using ELISA. MTT and Hoechst staining assay were applied to observe the inhibitory and killing effects of MDA-7 on the HCC cells. By using flow cytometry, the apoptosis, cell cycle and proliferation of SMMC-7721 and L02 cells were measured. The results showed recombinant replication-incompetent virus expressing MDA-7/IL-24 was constructed successfully, and RT-PCR revealed that it could mediate the high expression of the exogenous gene MDA-7/IL-24 in SMMC-7721 and L02 cells. The expression of MDA-7/IL-24 proteins in the culture superuatant was detectable by ELISA. Ad.mda-7 infection induced apoptosis and growth suppression in SMMC-7721 cells and an increased percentage of HCC cells in the GyM phase of the cell cycle, but not in L02 cells. It was concluded that mda-7/IL-24 gene, mediated with replication-incompetent adenovirus vector, could selectively induce growth suppression and apoptosis in HCC cell line SMMC-7721 but without any toxic side-effect on normal liver line L02.
|
| 关 键 词： | 肝细胞癌 基因治疗 细胞增生 病理机制 |
| 收稿时间： | 19 September 2006 |
Replication-incompetent adenovirus vector-mediated MDA-7/IL-24 selectively induces growth suppression and apoptosis of hepatoma cell Line SMMC-7721 |
| |
| Congjun Wang,Xinbo Xue,Jilin Yi,Zaide Wu,Kun Chen,Jianwei Zheng,Wenwei Ji,Yuan Yu.Replication-incompetent Adenovirus Vector-mediated MDA-7/IL-24 Selectively Induces Growth Suppression and Apoptosis of Hepatoma Cell Line SMMC-7721[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2008,28(1):80-83. |
| |
| Authors: | Congjun Wang Xinbo Xue Jilin Yi Zaide Wu Kun Chen Jianwei Zheng Wenwei Ji Yuan Yu |
| |
| Institution: | Department of Biliary and Pancreatic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
| |
| Abstract: | In order to investigate the effect of replication-incompetent adenovirus vector expressing MDA-7/IL-24 on tumor growth and apoptosis of human hepatocellular carcinoma (HCC) cell line SMMC-7721 and normal liver cell line L02, the recombinant replication-incompetent Ad.mda-7 virus vector was constructed and infected into the HCC cell line SMMC-7721 and normal liver cell line L02. RT-PCR was performed to examine the expression of MDA-7 mRNA. The concentrations of MDA-7/IL-4 in culture supernatants were determined by using ELISA. MTT and Hoechst staining assay were applied to observe the inhibitory and killing effects of MDA-7 on the HCC cells. By using flow cytometry, the apoptosis, cell cycle and proliferation of SMMC-7721 and L02 cells were meas-ured. The results showed recombinant replication-incompetent virus expressing MDA-7/IL-24 was constructed successfully, and RT-PCR revealed that it could mediate the high expression of the ex-ogenous gene MDA-7/IL-24 in SMMC-7721 and L02 cells. The expression of MDA-7/IL-24 proteins in the culture supernatant was detectable by ELISA. Ad.mda-7 infection induced apoptosis and growth suppression in SMMC-7721 cells and an increased percentage of HCC cells in the G2/M phase of the cell cycle, but not in L02 cells. It was concluded that mda-7/IL-24 gene, mediated with replication-incompetent adenovirus vector, could selectively induce growth suppression and apoptosis in HCC cell line SMMC-7721 but without any toxic side-effect on normal liver line L02. |
| |
| Keywords: | cancer gene therapy hepatocellular carcinoma apoptosis growth suppression MDA-7/IL-24 |
| 本文献已被 CNKI 维普 万方数据 SpringerLink 等数据库收录！ |
|
