原发性胆汁肝硬化患者血清中抗线粒体抗体及其亚型和抗核抗体的联合检测的临床价值

为了分析原发性胆汁性肝硬化(primary biliary cirrhosis,PBC)患者的抗线粒体抗体(anti-mitochondria antibody,AMA)及其M2、M4、M9亚型、抗核抗体(antinuclear antibody,ANA)的阳性率。应用间接免疫荧光法检测ANA、AMA,免疫斑点法检测AMA M2、M4、M9。结果显示91例PBC患者中AMA有89例为阳性。其中96.7%(88/91)的患者M2型阳性,45.1%(41/91)M4型阳性,2.2%(2/91)M9型阳性。39/91例患者ANA阳性,其中21例为核膜型。ANA、AMA及其M2、M4、M9亚型联合检测对于PBC患者的诊断有重要价值。

Detection of the anti-mitochondrial antibody,its subtypes and the anti-nuclear in sera of patients with primary biliary cirrhosis

The anti-mitochondrial antibody(AMA) and the anti-nuclear antibody(ANA) in sera of 91 patients with primary biliary cirrhosis(PBC) were detected by indirect immunofluorescence assay,and the immunoblot assay was used to detect the subtypes of AMA.It was found that in the sera of these patients,97.8%(89/91) of patients with PBC was positive for AMA,in which 96.7%(88/91),45.1%(4/91) and 2.2%(2/91) were positive for subtype M2,M4 and M9 of AMA respectively.Thirty nine patients out of 91 patients with PBC(42.9%) were positive for ANA,in which 21 patients showed nuclear membranous pattern.It is concluded the combined detectioin of AMA,its subtypes and ANA may be important for the diagnosis of primary biliary cirrhosis.