miR-34a对鼻咽癌细胞增殖的负性调控作用

目的:研究miR-34a对鼻咽癌细胞的增殖能力的影响。方法:使用PCR检测正常鼻咽上皮细胞及鼻咽癌细胞中miR-34a的表达水平,瞬时转染后通过MTT、克隆形成实验及凋亡实验探讨miR-34a对鼻咽癌细胞生物学行为的影响。结果:miR-34a在鼻咽癌细胞中表达量低于正常细胞,miR-34a抑制鼻咽癌细胞增殖及克隆形成能力,诱导凋亡。

Inhibitory Effect of MiR-34a on the Proliferation of Nasopharyngeal Cancer Cells

Objective:To investigate the effect of miR-34a on the growth of nasopharyngeal cancer cells in vitro.Methods:The expression of miR-34a both in normal nasopharyngeal epithelium and nasopharyngeal cancer cell lines were assayed by quantitative PCR.MTT assay,clone formation assay and flow cytometry were also applied to study the effect of miR-34a on nasopharyngeal cancer cells.Results:miR-34a expression was decreased in nasopharyngeal cancer cell than in normal nasopharyngeal epithelial cell.miR-34a up-regulation contributed to a dramatically reduction of proliferation and clone formation while it also showed an increasing induction of apoptosis in CNE1 and CNE2 cell lines.Conclusion:miR-34a expression was downregulated in nasopharyngeal cancer cells and its overexpression in vitro resulted in a greatly reduction of cell proliferation as well as a promotion of apoptosis in nasopharyngeal cancer cells.