挑筋法诱导佐剂型关节炎大鼠滑膜细胞凋亡的实验研究

目的:实验研究挑筋法对佐剂型关节炎大鼠滑膜细胞凋亡影响。 方法:将40只SD大鼠随机分为正常组、模型组、药物组、挑筋组,每组10只,实验中建立佐剂型关节炎模型,运用TUNEL法检测挑筋法对大鼠滑膜细胞凋亡的影响。 结果:致炎后模型组大鼠后踝关节红、肿,大鼠跛行,体质量下降,跖围与体质量分别为(25.48±2.16)mm,(123.4±12.8)g,经挑筋疗法治疗后大鼠后踝关节红、肿消失,跖围、体质量分别为(22.15±1.17)mm,(132.5±14.2)g,与模型组比较差异有显著性意义(P<0.001)。运用TUNEL检测各组滑胞细胞凋亡发现挑筋组和药物组出现明显的细胞凋亡,两者凋亡率分别为(43.81±3.84)％,(51.58±9.73)％,两者差异有显著性意义(P<0.05),药物组细胞凋亡率高于挑筋组。 结论:挑筋法诱导佐剂型关节炎大鼠滑膜细胞凋亡,故可能通过抑制和减少滑膜细胞的过度增生治疗炎性疾病。

Experimental study on the effect of fibrous-tissue-broken pricking on apoptosis of rat synovium in Freund''''s adjuvant arthritis

AIM:To study the effect of fibrous-tissue-broken pricking on apoptosis of rat synovium in Freund's adjuvant arthritis. METHODS:A total of 40 SD rats were divided into normal group,model group,medication group,and pricking group,with 10 rats in each group. Freund's adjuvant arthritis model was established and the effect of fibrous-tissue-broken pricking on apoptosis in rat synovium was detected using TUNEL method. RESULTS:The inflammatory rats in model group limped with swollen posterior ankle joint and their body mass decreased,the width of plantar circumference and body mass of (25.48± 2.16) mm and (123.4± 12.8) g,respectively.After the treatment by fibrous-tissue-broken pricking, the swelling relieved,with plantar circumference and body mass of (22.15± 1.17) mm and (132.5± 14.2) g,respectively.There was a significant difference compared with the model group(P< 0.001).TUNEL detection revealed that the apoptosis index of the medication group ( 51.58± 9.73)% was higher than that of the pricking group (43.81± 3.84)% with a significant difference(P< 0.05),in spite of remarkable cell apoptosis in both groups. CONCLUSION:Fibrous-tissue-broken pricking can induce apoptosis of rat synovium in Freund's adjuvant arthritis.Therefore,inflammatory diseases may be treated through suppressing and reducing the overproliferation of synovium.