抗增殖蛋白过表达对HepG2细胞增殖和凋亡的影响*

目的 探讨抗增殖蛋白（PHB）过表达对人肝癌细胞系HepG₂细胞增殖和凋亡的影响。方法 在已构建的PHB真核表达质粒pEGFP-N1-PHB瞬时转染HepG₂细胞,采用四甲基偶氮唑盐比色法（MTT法）检测HepG₂细胞增殖;使用流式细胞仪检测HepG₂细胞周期和凋亡。结果 转染pEGFP-N1-PHB细胞增殖明显低于pEGFP-N1空载转染细胞或未转染细胞（P<0.05）;在转染后48 h,转染组G2/M期细胞比例为（27.84±0.47）%,显著高于空载转染组的（17.21±0.64）%或未转染组的（22.67±0.33）%（P<0.001）;转染组细胞凋亡率为（31.72±0.35）%,显著高于空载转染组的（18.66±0.56）%或未转染组的（13.47±0.94）%（P<0.001）。结论 PHB过表达可抑制人肝癌HepG₂细胞增殖,诱导细胞进入G2/M期后被阻滞,并诱导细胞凋亡。

Effect of prohibitin over-expression on proliferation and apoptosis of human hepatocellular carcinoma cell line HepG2 cells

Objective To investigate the effect of prohibitin（PHB） over-expression on proliferation and apoptosis of human hepatocellular carcinoma cell line HepG2 cells. Methods The eukaryotic expression plasmid （pEGFP-N1-PHB） had been constructed and the HepG2 cells were transiently transfected with recombinant plasmid. Measurement of cell proliferation was performed by using MTT assay. Flow cytometry was carried out to detect cell cycle alterations and apoptosis. Results The cell growth curves showed that the proliferation of HepG2 cells were significantly decreased after transfection with pEGFP-N1-PHB as compared with pEGFP-N1 or controls（P<0.05）;At 48 hours after transfection,the proportion of G2/M phase of HepG2 cells in pEGFP-N1-PHB-transfected group was（27.84±0.47）%,significantly higher than in pEGFP-N1-transfected group[（17.21± 0.64）%] or in the non-transfected group[（22.67±0.33）%,P<0.001];The percentage of apoptotic cells in pEGFP-N1-PHB-transfected group was（31.72±0.35）%,much higher than（18.66±0.56） % in pEGFP-N1-transfected or non-transfected cells [（13.47±0.94）%,P<0.001]. Conclusion PHB overexpression might inhibit the proliferation, induce G2/M phase arrest and apoptosis of HepG2 cells.