白细胞介素-18在类风湿关节炎患者血清中的表达及临床分析

目的 研究白细胞介素18(interleukin-18,IL-18)在类风湿关节炎(RA)患者血清中的表达水平,并进一步探讨它们与临床指标的关系.方法 将63例RA患者组分为抗CCP阳性组和阴性组,应用ELISA方法检测63例患者和27例对照组的IL-18表达水平.结果 IL-18在RA抗CCP阳性组、阴性组和对照组依次为213.27±49.60pg/ml、192.03±32 88pg/ml和185.42±44.93pg/ml,抗CCP阳性组的表达水平高于抗CCP阴性组和对照组,其差异有统计学意义(P<0.01).RA抗CCP阳性组患者IL-18水平与DAS28呈负相关(r=-0.28,P<0.05),与外周血WBC计数呈负相关性(r=-0.51,P<0.05),而与ESR、CRP无相关性(P>0.05).RA抗CCP阴性组患者IL-18水平与DAS28呈负相关(P<0.05).结论 IL-18水平在RA患者表达升高,IL-18水平与DAS28呈负相关推测IL-18可能参与了RA的发病过程.

Expression and Clinical Significance of IL-18 in Rheumatoid Arthritis Patients

ObjectiveTo investigate the expression of sera interleukin-18 (IL-18), and analyzs its clinical significance. Methods63 rheumatoid arthritis(RA) patients were divided into two groups according to anti-cyclic citrullinated peptide (anti-CCP) antibodies,and 27 normal subjects were enrolled as control group. Levels of IL-18 in the serum were analysed by ELISA.ResultsIn anti-CCP antibodies positive patients, IL-18 serum levels were enhanced compared with two other groups and the levels of expression were 213.27±49.60pg/ml,192.03±32.88pg/ml and 185.42±44.93pg/ml, respectively among the three groups, and this changes were significant (P<0.01). In anti-CCP positive RA patients, elevated IL-18 level was negative correlated with DAS28 and WBC counts. Plasma levels of IL-18 showed no correlation with ESR and CRP. ConclusionIL-18 level was negative correlated with DAS28. IL-18 appears to be involved in the pathogenetic stage of RA.