晚期肺癌患者调节性T细胞和IL-10表达的变化

探讨晚期肺癌患者的CD4＋CD25＋调节性T细胞（Treg）、IL-10及其他T细胞亚群表达的临床意义。检测晚期肺癌患者92例和正常对照者64名的IL-10（ELISA法）、CD4＋CD25＋调节性T细胞及其他T细胞亚群（流式细胞仪法）。结果显示：肺癌组血清IL-10明显大于对照组（278±34ng/L vs 122±65ng/L,P〈0.01）、CD4＋CD25＋Treg细胞明显大于对照组（17.8±5.2）%vs（7.1±0.4）%,P〈0.01]、CD3＋、CD4＋、CD8＋CD28＋和NK细胞明显小于对照组（58.4±7.8）%vs（78.2±6.4）%,P≤0.05;（34.4±7.6）%vs（44.9±8.4）%,P〈0.01;（9.4±3.6）%vs（16.5±2.7）%,P〈0.01;（9.4±3.6）%vs（18.5±7.2）%,P〈0.01]。CD8＋T细胞明显高于对照组（37.8±6.5）%vs（31.8±5.1）%,P〈0.01]。CD4＋CD25＋Treg细胞、IL-10与CD8＋CD28＋细胞、NK细胞呈明显负相关。这些结果表明,CD4＋CD25＋Treg细胞与IL-10增多为晚期肺癌患者免疫功能受损的表现。

Changes of Serum Regulatory T Cells and IL-10 in Patients with Advanced Lung Cancer

To explore the clinical significance of the expression of CD4+CD25+ regulatory T cells,IL-10 and other T cell subsets in blood of patients with advanced lung cancer,serum levels of IL-10 in 92 patients with advanced lung cancer and 62 normal controls were measured by ELISA.CD4+CD25+ regulatory T cells other T cell subsets(CD3+,CD4+,CD8+ and CD8+CD28+) and NK cells were measured by flow cytometry.The results showed that the serum IL-10 levels in patients were significantly higher than that in control group(P<0.01).The percentage of CD4+CD25+ regulatory T cells in patients increased significantly than that in the control group(P<0.01).The percentage of CD3+cells,CD4+cells,CD8+CD28+ cells and NK cells in patients decreased significantly compared with those in the control group(P<0.01).The percentage of CD8+cells in patients significantly increased compared with that in the control group(P<0.01).There was significant negative correlation between CD4+CD25+ regulatory T cell IL-10 and CD8+CD28+ cell and NK cell.The results indicate that the increased levels of CD4+CD25+regulatory T cell and IL-10 may impair immune function in patients with advanced lung cancer.