涎腺良性多形性腺瘤和恶性多形性腺瘤中C—erbB—2和bc1—2蛋白产物的表达及其意义

为了探讨细胞增殖和细胞凋亡的调控基因在涎腺肿瘤的发生发展及恶性转变中的作用及其意义。本文采用免疫组化方法对5例瘤旁腺体、21例良性多形性腺瘤和24例恶性多形性腺瘤中C—erbB—2和bcl—2蛋白产物的表达进行形态学观察。结果表示:C—erbB—2和bcl—2在良性多形性腺瘤中均过度表达,表达率分别为29%和19%,主要分布于腺管样结构的腺上皮细胞的胞浆中。恶性多形性腺瘤中C—erbB—2的表达率为58%,主要分布于腺癌细胞的胞膜;bcl—2表达率为46%,主要分布于腺癌细胞的核膜及部分胞浆中。5例瘤旁腺体内导管上皮细胞也存在C—erbB—2和bcl—2的过度表达。结果表明:C—erbB—2蛋白产物的过度表达在涎腺多形性腺瘤的发生发展及恶性转变中起着重要的作用,同时也表明在涎腺多形性腺瘤发生中不仅存在细胞增殖基因调控失常同时也存在着细胞凋亡基因的调控失常,它们协同作用共同参与了涎腺多形性腺瘤的发生发展及恶性转化。

Expression of C-erbB-2 and Bcl-2 protein in benign pleomorphic adenomas and malignant pleomorphic adenomas of salivary gland

Expression of C-erbB-2 and bcl-2 protein were detected immunohistochemically in 21 cases of benign pleomorphic adenomas and 24 cases of malignant pleomorphic adenomas of salivany gland. The results showed that C-erbB-2 and bcl-2 were overexpressed in benign pleomorphic adenomas. The positive rates of C-erbB-2 and bcl-2 in benign pleomorphic adenomas were 29% and 19% respectively. The positive staining mainly rested on the cytoplasms of grandular epithelial cells in the tubular structures. The positive rates of C-erbB-2 and bcl-2 in malignant pleomorphic adenomas were 58% and 46% respectively . The positive staining of C-erbB-2 protein mainly existed on membrane of adenocarcinoma cells,bcl-2 protein mainly distributed on neclei membrane and cytoplasms of adenocatcinoma cells. neclei membrane and cytoplasms of carcinoma cells. The salivary gland ductal epithelial cells near tumors were positive for C-erbB-2 and bcl-2. The results indicated that overexpression of C-erbB-2 protein played an important role in the tumorigenesis, development and transformation of salivary gland tumors. It further indicated that cell apoptosis is also one of the reasons in the tumorigenesis of salivary gland.