| 杜氏盐藻14-3-3蛋白cDNA片段的克隆与分析 |
| |
| 引用本文： | 姬翔,王天云,王亚峰,刘红涛,王建民,薛乐勋.杜氏盐藻14-3-3蛋白cDNA片段的克隆与分析[J].郑州大学学报(医学版),2006,41(3):433-435. |
| |
| 作者姓名： | 姬翔 王天云 王亚峰 刘红涛 王建民 薛乐勋 |
| |
| 作者单位： | 1. 郑州大学细胞生物学研究室,郑州大学第一附属医院临床医学重点学科开放实验室,郑州,450052
2. 郑州大学细胞生物学研究室,郑州大学第一附属医院临床医学重点学科开放实验室,郑州,450052;新乡医学院生物化学与分子生物学教研室,新乡,453003 |
| |
| 基金项目： | 中国科学院资助项目
,
河南省医学科技人才创新工程项目 |
| |
| 摘 要： | 目的:克隆杜氏盐藻14-3-3蛋白的cDNA片段,并对其进行测序和序列分析.方法:根据衣藻、烟草、豌豆、拟南芥、西红柿等14-3-3蛋白的高度保守序列SVAYKNV、DSTLIMQ设计一对简并引物,采用RT-PCR方法扩增杜氏盐藻14-3-3蛋白的cDNA片段.PCR产物克隆至T载体后转化大肠杆菌JM109,筛选阳性菌落,测序,BLAST进行同源性比对.结果:克隆获得531 bp的cDNA片段,编码177个氨基酸(GenBank AN:AY965896).同源性比较显示,序列与其他生物具有高度的同源性,其与衣藻、烟草、豌豆、拟南芥、西红柿、人等的同源性分别为:94%,84%,84%,83%,83%和80%.结论:克隆得到了杜氏盐藻14-3-3蛋白的cDNA片段.
|
| 关 键 词： | 杜氏盐藻 14-3-3蛋白 cDNA 简并引物 |
| 收稿时间： | 2005-11-15 |
| 修稿时间： | 2005年11月15 |
Cloning and analysis of 14-3-3 protein cDNA fragment of Dunaliella salina |
| |
| JI Xiang,WANG Tianyun,WANG Yafeng,LIU Hongtao,WANG Jianmin,XUE Lexun.Cloning and analysis of 14-3-3 protein cDNA fragment of Dunaliella salina[J].Journal of Zhengzhou University: Med Sci,2006,41(3):433-435. |
| |
| Authors: | JI Xiang WANG Tianyun WANG Yafeng LIU Hongtao WANG Jianmin XUE Lexun |
| |
| Institution: | 1, Laboratory for Cell Biology ; Key Laboratory for Clinical Medicine, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052 ;2,Department of Biochemistry and Molecular Biology, Xinxiang Medical College, Xinxiang 453003 |
| |
| Abstract: | Aim: To clone and identify 14-3-3 protein cDNA fragment from Dunaliella salina.Methods:A pair of degenerate primers was designed according to the conserved motifs of SVAYKNV and DSTLIMQ of homologous amino acid sequences, and total RNA of Dunaliella salina was extracted using TRIzol reagent. A cDNA fragment obtained through RT-PCR was inserted into T-vector and then transformed into E. coli JM109. Several positive colonies were randomly selected and screened for sequencing. Homologous analysis of the deduced amino acid sequences was performed by BLAST and subsequently compared with those of GenBank. Results: The cloned cDNA sequence was 531bp, which encoded 177 amino acids(GenBank accession number:AY965896).The sequence shared high identity with the following 14-3-3 proteins: Chlamydomonas reinhardtii 94%, Nicotiana tabacum 84%, Pisum sativum 84%, Arabidopsis thaliana 83%, tomato 83%, and human beings 80%, respectively.Conclusion: The cloned sequence is 14-3-3 cDNA fragment from Dunaliella salina. |
| |
| Keywords: | Dunaliella salina 14-3-3 protein cDNA degenerate primer |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
